Multidrug resistance in Enterococcus species of faecal origin from commercial dairy lactating cattle: Public health concern

Godfred Ngu Tanih, Anthony Ifeanyin Okoh, Roland Ndip Ndip, Uchechukwu Uchechukwu Nwodo, Ezekiel Green

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Objective: To evaluate the prevalence of Enterococcus species in cattle faeces, their corresponding drug resistant patterns as well as the genes coding for resistance in the isolates. Methods: Two hundred and ninety rectal swabs were cultured for the isolation of Enterococcus. Presumptive isolates were confirmed by PCR, targeting the tuf gene, and confirmed isolates were identified to species level, using species-specific primers aimed at targeting six different species. Additionally, antibiogram was performed by disc diffusion and genes implicated in resistance were evaluated using molecular methods. Results: All presumptive isolates were confirmed as Enterococcus and speciated as: Enterococcus hirae (82%), Enterococcus faecium (5%), Enterococcus durans (5%), Enterococcus faecalis (2%) and 6% of unidentified species. Resistance to various antimicrobials ranged from 16.4% for penicillin to 69.6% for erythromycin. Among the tetracycline and erythromycin-resistant isolates, tet M (100%) and erm B (29%) were the only amplified genes known to mediate resistance respectively. Other detected genes included van B (25%), van C1 (21%) and bla Z (11%). Conclusions: A high prevalence of multidrug resistant Enterococcus species was observed in this study, accentuating the need to improve on animal farming practices to prevent the dissemination of this microorganism to the environment.

Original languageEnglish
Pages (from-to)665-670
Number of pages6
JournalAsian Pacific Journal of Tropical Disease
Issue number11
Publication statusPublished - 1 Nov 2017


  • Antimicrobial resistance
  • Commercial dairy farms
  • Enterococcus
  • Resistant gene

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases


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