MS-DIAL and MS-FINDER-assisted dereplication of the edible mushroom, Phlebopus beniensis crude extract and fractions: Chemophenetic significance

The edible mushroom Phlebopus beniensis, was extracted, fractionated and the chemical profiles of the obtained fractions were established by dereplication of UPLC-ESI-QTOF-MS data assisted by MS-Dial and MS-Finder databases. Among the 35 detected secondary metabolites, sphingolipids, glycerophospolipids, a cerebroside, an iridoid, a lipophosphocholine, tetronic acid derivatives, and a pyrrole alkaloid were identified as main components. The identified lipids derivatives and part of these tetronic acids are herein reported for the first time in P. beniensis. The crude extract and fractions were evaluated for their inhibitory effect against the Trypanosoma cruzi enzyme, trypanothione reductase (TryTR). The n-butanol fraction showed inhibitory percentages of 99.0 ± 1.8 % (corresponding to the IC₅₀ value of 23.2 ± 7.3 μg/mL) and was more active than the crude extract (inhibitory effect of 4.8 ± 3.3%). The chemophenetic significances of the identified compounds were discussed.