Modelling of the impedimetric responses of an aflatoxin B1 immunosensor prepared on an electrosynthetic polyaniline platform

Joseph H.O. Owino; Anna Ignaszak; Amir Al-Ahmed; Priscilla G.L. Baker; Hailemichael Alemu; Jane Catherine Ngila; Emmanuel I. Iwuoha

doi:10.1007/s00216-007-1333-9

Modelling of the impedimetric responses of an aflatoxin B₁ immunosensor prepared on an electrosynthetic polyaniline platform

Joseph H.O. Owino, Anna Ignaszak, Amir Al-Ahmed, Priscilla G.L. Baker, Hailemichael Alemu, Jane Catherine Ngila, Emmanuel I. Iwuoha

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

Aflatoxins are a group of mycotoxins that have deleterious effects on humans and are produced during fungal infection of plants or plant products. An electrochemical immunosensor for the determination of aflatoxin B₁ (AFB₁) was developed with AFB₁antibody (AFB ₁-Ab) immobilized on Pt electrodes modified with polyaniline (PANi) and polystyrene sulphonic acid (PSSA). Impedimetric analysis shows that the electron transfer resistances of the Pt/PANi-PSSA electrode, the Pt/PANi-PSSA/AFB₁-Ab immunosensor and Pt/PANi-PSSA/AFB₁-Ab incubated in bovine serum albumin (BSA) were 0.458, 720 and 1,066kΩ, respectively. These results indicate that electrochemical impedance spectroscopy (EIS) is a suitable method for monitoring the change in electron transfer resistance associated with the immobilization of the antibody. Modelling of EIS data gave equivalent circuits which showed that the electron transfer resistance increased from 0.458 kΩ for the Pt/PANi-PSSA electrode to 1,066kΩ for the Pt/PANi-PSSA/AFB₁-Ab immunosensor, indicating that immobilization of the antibody and incubation in BSA introduced an electron transfer barrier. The AFB₁ immunosensor had a detection limit of 0.1 mg/L and a sensitivity of 869.6 kΩ L/mg.

Original language	English
Pages (from-to)	1069-1074
Number of pages	6
Journal	Analytical and Bioanalytical Chemistry
Volume	388
Issue number	5-6
DOIs	https://doi.org/10.1007/s00216-007-1333-9
Publication status	Published - Jul 2007
Externally published	Yes

Keywords

Aflatoxin B
Aflatoxin B antibody
Differential pulse voltammetry
Electrochemical impedance spectroscopy
Immunosensor

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

Access to Document

10.1007/s00216-007-1333-9

Cite this

@article{961f46ac833e499e84285ce50ce99acb,

title = "Modelling of the impedimetric responses of an aflatoxin B1 immunosensor prepared on an electrosynthetic polyaniline platform",

abstract = "Aflatoxins are a group of mycotoxins that have deleterious effects on humans and are produced during fungal infection of plants or plant products. An electrochemical immunosensor for the determination of aflatoxin B1 (AFB1) was developed with AFB1antibody (AFB 1-Ab) immobilized on Pt electrodes modified with polyaniline (PANi) and polystyrene sulphonic acid (PSSA). Impedimetric analysis shows that the electron transfer resistances of the Pt/PANi-PSSA electrode, the Pt/PANi-PSSA/AFB1-Ab immunosensor and Pt/PANi-PSSA/AFB1-Ab incubated in bovine serum albumin (BSA) were 0.458, 720 and 1,066kΩ, respectively. These results indicate that electrochemical impedance spectroscopy (EIS) is a suitable method for monitoring the change in electron transfer resistance associated with the immobilization of the antibody. Modelling of EIS data gave equivalent circuits which showed that the electron transfer resistance increased from 0.458 kΩ for the Pt/PANi-PSSA electrode to 1,066kΩ for the Pt/PANi-PSSA/AFB1-Ab immunosensor, indicating that immobilization of the antibody and incubation in BSA introduced an electron transfer barrier. The AFB1 immunosensor had a detection limit of 0.1 mg/L and a sensitivity of 869.6 kΩ L/mg.",

keywords = "Aflatoxin B, Aflatoxin B antibody, Differential pulse voltammetry, Electrochemical impedance spectroscopy, Immunosensor",

author = "Owino, {Joseph H.O.} and Anna Ignaszak and Amir Al-Ahmed and Baker, {Priscilla G.L.} and Hailemichael Alemu and Ngila, {Jane Catherine} and Iwuoha, {Emmanuel I.}",

year = "2007",

month = jul,

doi = "10.1007/s00216-007-1333-9",

language = "English",

volume = "388",

pages = "1069--1074",

journal = "Analytical and Bioanalytical Chemistry",

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T1 - Modelling of the impedimetric responses of an aflatoxin B1 immunosensor prepared on an electrosynthetic polyaniline platform

AU - Owino, Joseph H.O.

AU - Ignaszak, Anna

AU - Al-Ahmed, Amir

AU - Baker, Priscilla G.L.

AU - Alemu, Hailemichael

AU - Ngila, Jane Catherine

AU - Iwuoha, Emmanuel I.

PY - 2007/7

Y1 - 2007/7

N2 - Aflatoxins are a group of mycotoxins that have deleterious effects on humans and are produced during fungal infection of plants or plant products. An electrochemical immunosensor for the determination of aflatoxin B1 (AFB1) was developed with AFB1antibody (AFB 1-Ab) immobilized on Pt electrodes modified with polyaniline (PANi) and polystyrene sulphonic acid (PSSA). Impedimetric analysis shows that the electron transfer resistances of the Pt/PANi-PSSA electrode, the Pt/PANi-PSSA/AFB1-Ab immunosensor and Pt/PANi-PSSA/AFB1-Ab incubated in bovine serum albumin (BSA) were 0.458, 720 and 1,066kΩ, respectively. These results indicate that electrochemical impedance spectroscopy (EIS) is a suitable method for monitoring the change in electron transfer resistance associated with the immobilization of the antibody. Modelling of EIS data gave equivalent circuits which showed that the electron transfer resistance increased from 0.458 kΩ for the Pt/PANi-PSSA electrode to 1,066kΩ for the Pt/PANi-PSSA/AFB1-Ab immunosensor, indicating that immobilization of the antibody and incubation in BSA introduced an electron transfer barrier. The AFB1 immunosensor had a detection limit of 0.1 mg/L and a sensitivity of 869.6 kΩ L/mg.

AB - Aflatoxins are a group of mycotoxins that have deleterious effects on humans and are produced during fungal infection of plants or plant products. An electrochemical immunosensor for the determination of aflatoxin B1 (AFB1) was developed with AFB1antibody (AFB 1-Ab) immobilized on Pt electrodes modified with polyaniline (PANi) and polystyrene sulphonic acid (PSSA). Impedimetric analysis shows that the electron transfer resistances of the Pt/PANi-PSSA electrode, the Pt/PANi-PSSA/AFB1-Ab immunosensor and Pt/PANi-PSSA/AFB1-Ab incubated in bovine serum albumin (BSA) were 0.458, 720 and 1,066kΩ, respectively. These results indicate that electrochemical impedance spectroscopy (EIS) is a suitable method for monitoring the change in electron transfer resistance associated with the immobilization of the antibody. Modelling of EIS data gave equivalent circuits which showed that the electron transfer resistance increased from 0.458 kΩ for the Pt/PANi-PSSA electrode to 1,066kΩ for the Pt/PANi-PSSA/AFB1-Ab immunosensor, indicating that immobilization of the antibody and incubation in BSA introduced an electron transfer barrier. The AFB1 immunosensor had a detection limit of 0.1 mg/L and a sensitivity of 869.6 kΩ L/mg.

KW - Aflatoxin B

KW - Aflatoxin B antibody

KW - Differential pulse voltammetry

KW - Electrochemical impedance spectroscopy

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