Abstract
Serial blood sampling involving sampling blood from the same individual at different time points is essential in time-based studies including xenobiotic toxicokinetics and biochemical studies. However, high fish mortality due to phlebotomy-induced anaemia (PIA) constrains serial blood sampling in small to medium sized fish. The aim of the study was to develop and implement a model-based serial blood sampling protocol that minimises fish mortality by regulating anaemia within levels that sustain fish survival and recovery. A model simulating the reduction in haemoglobin was developed from blood sampling data of sixteen (N=16) medium sized Oreochromis mossambicus. The model was incorporated into a serial blood sampling protocol whose performance was tested on eight (N=8) fish. The protocol avoided fish mortality and the fish recovered from PIA within three weeks of the post-sampling period. Therefore, managing anaemia minimises mortality and improves the applicability of serial blood sampling in small to medium sized fish.
Original language | English |
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Journal | Biology Open |
Volume | 7 |
Issue number | 12 |
DOIs | |
Publication status | Published - 2018 |
Keywords
- Fish
- Model-based
- Serial blood sampling
ASJC Scopus subject areas
- General Biochemistry,Genetics and Molecular Biology
- General Agricultural and Biological Sciences