Mixed infection of virus and phytoplasma in gladiolus varieties in India

Tasou Rihne; Manish Kumar; Yapalahalli Sathyanarayanappa Shreenath; Rajendra Prasad Pant; Aido Taloh; Kishan Swaroop; Govind Pratap Rao

doi:10.5958/2249-4677.2019.00075.6

Mixed infection of virus and phytoplasma in gladiolus varieties in India

Tasou Rihne, Manish Kumar, Yapalahalli Sathyanarayanappa Shreenath, Rajendra Prasad Pant, Aido Taloh, Kishan Swaroop, Govind Pratap Rao

ICAR - Indian Agricultural Research Institute

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Gladiolus grandiflorus is a bulbous ornamental plant popularly grown for its cut flowers of high commercial value. On a survey to the experimental fields at IARI, New Delhi, during 2016-2018. About 8% to 96 % disease incidence of leaf yellowing and streaks were observed in eleven varieties of gladiolus. The presence of ‘Candidatus Phytoplasma trifolii’ (16SrVI-D) subgroup in all the eleven symptomatic varieties was confirmed by PCR amplification using phytoplasma specific primer pairs P1/P7 and R16F2n/R16R2 and amplified 16Sr DNA sequence comparison. The presence of mixed infection with Cucumber mosaic virus (CMV) was also confirmed by electron microscopy and coat protein sequence analysis of gladioli samples. The results suggest the presence of mixed infection of 16SrI-D subgroup phytoplasmas and CMV associated with leaf yellowing and streaks symptoms in gladiolus varieties.

Original language	English
Pages (from-to)	149-150
Number of pages	2
Journal	Phytopathogenic Mollicutes
Volume	9
Issue number	1
DOIs	https://doi.org/10.5958/2249-4677.2019.00075.6
Publication status	Published - Jun 2019
Externally published	Yes

Keywords

16SrVI-D subgroup
Cucumber mosaic virus
Gladiolus
Phytoplasma

ASJC Scopus subject areas

Parasitology
Ecology, Evolution, Behavior and Systematics
Microbiology (medical)
Cell Biology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.5958/2249-4677.2019.00075.6

Cite this

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title = "Mixed infection of virus and phytoplasma in gladiolus varieties in India",

abstract = "Gladiolus grandiflorus is a bulbous ornamental plant popularly grown for its cut flowers of high commercial value. On a survey to the experimental fields at IARI, New Delhi, during 2016-2018. About 8% to 96 % disease incidence of leaf yellowing and streaks were observed in eleven varieties of gladiolus. The presence of {\textquoteleft}Candidatus Phytoplasma trifolii{\textquoteright} (16SrVI-D) subgroup in all the eleven symptomatic varieties was confirmed by PCR amplification using phytoplasma specific primer pairs P1/P7 and R16F2n/R16R2 and amplified 16Sr DNA sequence comparison. The presence of mixed infection with Cucumber mosaic virus (CMV) was also confirmed by electron microscopy and coat protein sequence analysis of gladioli samples. The results suggest the presence of mixed infection of 16SrI-D subgroup phytoplasmas and CMV associated with leaf yellowing and streaks symptoms in gladiolus varieties.",

keywords = "16SrVI-D subgroup, Cucumber mosaic virus, Gladiolus, Phytoplasma",

author = "Tasou Rihne and Manish Kumar and Shreenath, {Yapalahalli Sathyanarayanappa} and Pant, {Rajendra Prasad} and Aido Taloh and Kishan Swaroop and Rao, {Govind Pratap}",

year = "2019",

month = jun,

doi = "10.5958/2249-4677.2019.00075.6",

language = "English",

volume = "9",

pages = "149--150",

journal = "Phytopathogenic Mollicutes",

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TY - JOUR

T1 - Mixed infection of virus and phytoplasma in gladiolus varieties in India

AU - Rihne, Tasou

AU - Kumar, Manish

AU - Shreenath, Yapalahalli Sathyanarayanappa

AU - Pant, Rajendra Prasad

AU - Taloh, Aido

AU - Swaroop, Kishan

AU - Rao, Govind Pratap

PY - 2019/6

Y1 - 2019/6

N2 - Gladiolus grandiflorus is a bulbous ornamental plant popularly grown for its cut flowers of high commercial value. On a survey to the experimental fields at IARI, New Delhi, during 2016-2018. About 8% to 96 % disease incidence of leaf yellowing and streaks were observed in eleven varieties of gladiolus. The presence of ‘Candidatus Phytoplasma trifolii’ (16SrVI-D) subgroup in all the eleven symptomatic varieties was confirmed by PCR amplification using phytoplasma specific primer pairs P1/P7 and R16F2n/R16R2 and amplified 16Sr DNA sequence comparison. The presence of mixed infection with Cucumber mosaic virus (CMV) was also confirmed by electron microscopy and coat protein sequence analysis of gladioli samples. The results suggest the presence of mixed infection of 16SrI-D subgroup phytoplasmas and CMV associated with leaf yellowing and streaks symptoms in gladiolus varieties.

AB - Gladiolus grandiflorus is a bulbous ornamental plant popularly grown for its cut flowers of high commercial value. On a survey to the experimental fields at IARI, New Delhi, during 2016-2018. About 8% to 96 % disease incidence of leaf yellowing and streaks were observed in eleven varieties of gladiolus. The presence of ‘Candidatus Phytoplasma trifolii’ (16SrVI-D) subgroup in all the eleven symptomatic varieties was confirmed by PCR amplification using phytoplasma specific primer pairs P1/P7 and R16F2n/R16R2 and amplified 16Sr DNA sequence comparison. The presence of mixed infection with Cucumber mosaic virus (CMV) was also confirmed by electron microscopy and coat protein sequence analysis of gladioli samples. The results suggest the presence of mixed infection of 16SrI-D subgroup phytoplasmas and CMV associated with leaf yellowing and streaks symptoms in gladiolus varieties.

KW - 16SrVI-D subgroup

KW - Cucumber mosaic virus

KW - Gladiolus

KW - Phytoplasma

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DO - 10.5958/2249-4677.2019.00075.6

M3 - Article

AN - SCOPUS:85078822426

SN - 2249-4669

VL - 9

SP - 149

EP - 150

JO - Phytopathogenic Mollicutes

JF - Phytopathogenic Mollicutes

IS - 1

ER -

Mixed infection of virus and phytoplasma in gladiolus varieties in India

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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