TY - JOUR
T1 - Microfluidics for detection of exosomes and microRNAs in cancer
T2 - State of the art
AU - Mousavi, Seyed Mojtaba
AU - Amin Mahdian, Seyed Mohammad
AU - Ebrahimi, Mohammad Saeid
AU - Taghizadieh, Mohammad
AU - Vosough, Massoud
AU - Sadri Nahand, Javid
AU - Hosseindoost, Saereh
AU - Vousooghi, Nasim
AU - Javar, Hamid Akbari
AU - Larijani, Bagher
AU - Hadjighassem, Mahmoud Reza
AU - Rahimian, Neda
AU - Hamblin, Michael R.
AU - Mirzaei, Hamed
N1 - Publisher Copyright:
© 2022 The Author(s)
PY - 2022/6/14
Y1 - 2022/6/14
N2 - Exosomes are small extracellular vesicles with sizes ranging from 30–150 nanometers that contain proteins, lipids, mRNAs, microRNAs, and double-stranded DNA derived from the cells of origin. Exosomes can be taken up by target cells, acting as a means of cell-to-cell communication. The discovery of these vesicles in body fluids and their participation in cell communication has led to major breakthroughs in diagnosis, prognosis, and treatment of several conditions (e.g., cancer). However, conventional isolation and evaluation of exosomes and their microRNA content suffers from high cost, lengthy processes, difficult standardization, low purity, and poor yield. The emergence of microfluidics devices with increased efficiency in sieving, trapping, and immunological separation of small volumes could provide improved detection and monitoring of exosomes involved in cancer. Microfluidics techniques hold promise for advances in development of diagnostic and prognostic devices. This review covers ongoing research on microfluidics devices for detection of microRNAs and exosomes as biomarkers and their translation to point-of-care and clinical applications.
AB - Exosomes are small extracellular vesicles with sizes ranging from 30–150 nanometers that contain proteins, lipids, mRNAs, microRNAs, and double-stranded DNA derived from the cells of origin. Exosomes can be taken up by target cells, acting as a means of cell-to-cell communication. The discovery of these vesicles in body fluids and their participation in cell communication has led to major breakthroughs in diagnosis, prognosis, and treatment of several conditions (e.g., cancer). However, conventional isolation and evaluation of exosomes and their microRNA content suffers from high cost, lengthy processes, difficult standardization, low purity, and poor yield. The emergence of microfluidics devices with increased efficiency in sieving, trapping, and immunological separation of small volumes could provide improved detection and monitoring of exosomes involved in cancer. Microfluidics techniques hold promise for advances in development of diagnostic and prognostic devices. This review covers ongoing research on microfluidics devices for detection of microRNAs and exosomes as biomarkers and their translation to point-of-care and clinical applications.
KW - biomarkers
KW - cancer
KW - exosomes
KW - microRNA
KW - microfluidics
UR - http://www.scopus.com/inward/record.url?scp=85130363718&partnerID=8YFLogxK
U2 - 10.1016/j.omtn.2022.04.011
DO - 10.1016/j.omtn.2022.04.011
M3 - Review article
AN - SCOPUS:85130363718
SN - 2162-2531
VL - 28
SP - 758
EP - 791
JO - Molecular Therapy - Nucleic Acids
JF - Molecular Therapy - Nucleic Acids
ER -
