Abstract
A new spectrophotometric method for ascorbic acid determination has been described. Methylene blue (MB) being a water-soluble, non-toxic, cationic dye (λmax 660 nm), has been observed to be immobilized successfully in the Stem layer of a cationic micelle (cetyl trimethyl ammonium bromide, CTAB) through hydrophobic interaction. The immobilized dye (λmax 660 nm) is quantitatively reduced by ascorbic acid (AA) in the alkaline medium (pH > 8.5). The progress of the reaction and stepwise bleaching of the blue colour of the dye gives a quantitative measure of ascorbic acid concentration. A single aliquot of the micelle stabilized MB could be used for the determination of ascorbic acid in the range of 50-400 ppm.
| Original language | English |
|---|---|
| Pages (from-to) | 632-635 |
| Number of pages | 4 |
| Journal | Indian Journal of Chemistry - Section B Organic and Medicinal Chemistry |
| Volume | 40 |
| Issue number | 7 |
| Publication status | Published - 2001 |
| Externally published | Yes |
ASJC Scopus subject areas
- Pharmacology, Toxicology and Pharmaceutics (all)
- Organic Chemistry