Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)

Innocentia G. Erdogan; Lukhanyo Mekuto; Seteno K.O. Ntwampe; Elvis Fosso-Kankeu; Frans B. Waanders

doi:10.1016/j.dib.2019.104135

Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)

Innocentia G. Erdogan, Lukhanyo Mekuto, Seteno K.O. Ntwampe, Elvis Fosso-Kankeu, Frans B. Waanders

Chemical Engineering Technology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The metagenomic data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O'Kiep, Namaqualand, South Africa. Representative samples from the source (Orange River) to the point of use (O'Kiep), through a 150km DWSS used for drinking water distribution were analysed for bacterial content. PCR amplification of the 16S rRNA V1–V3 regions was undertaken using oligonucleotide primers 27F and 518R subsequent to DNA extraction. The PCR amplicons were processed using the illumina^® reaction kits as per manufactures guidelines and sequenced using the illumina^® MiSeq-2000, by means of MiSeq V3 kit. The data obtained was processed using a bioinformatics QIIME software with a compatible fast nucleic acid (fna) file. The raw sequences were deposited at the National Centre of Biotechnology (NCBI) and the Sequence Read Archive (SRA) database, obtaining accession numbers for each species identified.

Original language	English
Article number	104135
Journal	Data in Brief
Volume	25
DOIs	https://doi.org/10.1016/j.dib.2019.104135
Publication status	Published - Aug 2019

Keywords

16S rRNA gene
Drinking water supply system (DWSS)
Metagenomics
O'Kiep

ASJC Scopus subject areas

Multidisciplinary

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.dib.2019.104135

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@article{217ed6318e8343eba16aaaa04f6b8cdc,

title = "Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)",

abstract = "The metagenomic data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O'Kiep, Namaqualand, South Africa. Representative samples from the source (Orange River) to the point of use (O'Kiep), through a 150km DWSS used for drinking water distribution were analysed for bacterial content. PCR amplification of the 16S rRNA V1–V3 regions was undertaken using oligonucleotide primers 27F and 518R subsequent to DNA extraction. The PCR amplicons were processed using the illumina{\textregistered} reaction kits as per manufactures guidelines and sequenced using the illumina{\textregistered} MiSeq-2000, by means of MiSeq V3 kit. The data obtained was processed using a bioinformatics QIIME software with a compatible fast nucleic acid (fna) file. The raw sequences were deposited at the National Centre of Biotechnology (NCBI) and the Sequence Read Archive (SRA) database, obtaining accession numbers for each species identified.",

keywords = "16S rRNA gene, Drinking water supply system (DWSS), Metagenomics, O'Kiep",

author = "Erdogan, {Innocentia G.} and Lukhanyo Mekuto and Ntwampe, {Seteno K.O.} and Elvis Fosso-Kankeu and Waanders, {Frans B.}",

note = "Publisher Copyright: {\textcopyright} 2019 The Author(s)",

year = "2019",

month = aug,

doi = "10.1016/j.dib.2019.104135",

language = "English",

volume = "25",

journal = "Data in Brief",

issn = "2352-3409",

publisher = "Elsevier BV",

}

Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep). / Erdogan, Innocentia G.; Mekuto, Lukhanyo; Ntwampe, Seteno K.O. et al.
In: Data in Brief, Vol. 25, 104135, 08.2019.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa

T2 - Source (lower Orange River) to point-of-use (O'Kiep)

AU - Erdogan, Innocentia G.

AU - Mekuto, Lukhanyo

AU - Ntwampe, Seteno K.O.

AU - Fosso-Kankeu, Elvis

AU - Waanders, Frans B.

PY - 2019/8

Y1 - 2019/8

N2 - The metagenomic data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O'Kiep, Namaqualand, South Africa. Representative samples from the source (Orange River) to the point of use (O'Kiep), through a 150km DWSS used for drinking water distribution were analysed for bacterial content. PCR amplification of the 16S rRNA V1–V3 regions was undertaken using oligonucleotide primers 27F and 518R subsequent to DNA extraction. The PCR amplicons were processed using the illumina® reaction kits as per manufactures guidelines and sequenced using the illumina® MiSeq-2000, by means of MiSeq V3 kit. The data obtained was processed using a bioinformatics QIIME software with a compatible fast nucleic acid (fna) file. The raw sequences were deposited at the National Centre of Biotechnology (NCBI) and the Sequence Read Archive (SRA) database, obtaining accession numbers for each species identified.

AB - The metagenomic data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O'Kiep, Namaqualand, South Africa. Representative samples from the source (Orange River) to the point of use (O'Kiep), through a 150km DWSS used for drinking water distribution were analysed for bacterial content. PCR amplification of the 16S rRNA V1–V3 regions was undertaken using oligonucleotide primers 27F and 518R subsequent to DNA extraction. The PCR amplicons were processed using the illumina® reaction kits as per manufactures guidelines and sequenced using the illumina® MiSeq-2000, by means of MiSeq V3 kit. The data obtained was processed using a bioinformatics QIIME software with a compatible fast nucleic acid (fna) file. The raw sequences were deposited at the National Centre of Biotechnology (NCBI) and the Sequence Read Archive (SRA) database, obtaining accession numbers for each species identified.

KW - 16S rRNA gene

KW - Drinking water supply system (DWSS)

KW - Metagenomics

KW - O'Kiep

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DO - 10.1016/j.dib.2019.104135

M3 - Article

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SN - 2352-3409

VL - 25

JO - Data in Brief

JF - Data in Brief

M1 - 104135

ER -

Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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