Metagenomic data of free cyanide and thiocyanate degrading bacterial communities

Lukhanyo Mekuto; Seteno K.O. Ntwampe; John B.N. Mudumbi; Enoch A. Akinpelu; Maxwell Mewa-Ngongang

doi:10.1016/j.dib.2017.06.049

Metagenomic data of free cyanide and thiocyanate degrading bacterial communities

Lukhanyo Mekuto, Seteno K.O. Ntwampe, John B.N. Mudumbi, Enoch A. Akinpelu, Maxwell Mewa-Ngongang

Cape Peninsula University of Technology

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The data presented in this article contains the bacterial community structure of the free cyanide (CN^-) and thiocyanate (SCN^-) degrading organisms that were isolated from electroplating wastewater and synthetic SCN^- containing wastewater. PCR amplification of the 16S rRNA V1-V3 regions was undertaken using the 27F and 518R oligonucleotide primers following the metacommunity DNA extraction procedure. The PCR amplicons were processed using the illumina® reaction kits as per manufacturer׳s instruction and sequenced using the illumina® MiSeq-2000, using the MiSeq V3 kit. The data was processed using bioinformatics tools such as QIIME and the raw sequence files are available via NCBI׳s Sequence Read Archive (SRA) database.

Original language	English
Pages (from-to)	738-741
Number of pages	4
Journal	Data in Brief
Volume	13
DOIs	https://doi.org/10.1016/j.dib.2017.06.049
Publication status	Published - Aug 2017
Externally published	Yes

Keywords

16S rRNA gene
Cyanide degrading organisms
Metagenomics
Thiocyanate degrading organisms

ASJC Scopus subject areas

Multidisciplinary

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10.1016/j.dib.2017.06.049

Cite this

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title = "Metagenomic data of free cyanide and thiocyanate degrading bacterial communities",

abstract = "The data presented in this article contains the bacterial community structure of the free cyanide (CN-) and thiocyanate (SCN-) degrading organisms that were isolated from electroplating wastewater and synthetic SCN- containing wastewater. PCR amplification of the 16S rRNA V1-V3 regions was undertaken using the 27F and 518R oligonucleotide primers following the metacommunity DNA extraction procedure. The PCR amplicons were processed using the illumina{\textregistered} reaction kits as per manufacturer׳s instruction and sequenced using the illumina{\textregistered} MiSeq-2000, using the MiSeq V3 kit. The data was processed using bioinformatics tools such as QIIME and the raw sequence files are available via NCBI׳s Sequence Read Archive (SRA) database.",

keywords = "16S rRNA gene, Cyanide degrading organisms, Metagenomics, Thiocyanate degrading organisms",

author = "Lukhanyo Mekuto and Ntwampe, {Seteno K.O.} and Mudumbi, {John B.N.} and Akinpelu, {Enoch A.} and Maxwell Mewa-Ngongang",

note = "Publisher Copyright: {\textcopyright} 2017",

year = "2017",

month = aug,

doi = "10.1016/j.dib.2017.06.049",

language = "English",

volume = "13",

pages = "738--741",

journal = "Data in Brief",

issn = "2352-3409",

publisher = "Elsevier BV",

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TY - JOUR

T1 - Metagenomic data of free cyanide and thiocyanate degrading bacterial communities

AU - Mekuto, Lukhanyo

AU - Ntwampe, Seteno K.O.

AU - Mudumbi, John B.N.

AU - Akinpelu, Enoch A.

AU - Mewa-Ngongang, Maxwell

PY - 2017/8

Y1 - 2017/8

N2 - The data presented in this article contains the bacterial community structure of the free cyanide (CN-) and thiocyanate (SCN-) degrading organisms that were isolated from electroplating wastewater and synthetic SCN- containing wastewater. PCR amplification of the 16S rRNA V1-V3 regions was undertaken using the 27F and 518R oligonucleotide primers following the metacommunity DNA extraction procedure. The PCR amplicons were processed using the illumina® reaction kits as per manufacturer׳s instruction and sequenced using the illumina® MiSeq-2000, using the MiSeq V3 kit. The data was processed using bioinformatics tools such as QIIME and the raw sequence files are available via NCBI׳s Sequence Read Archive (SRA) database.

AB - The data presented in this article contains the bacterial community structure of the free cyanide (CN-) and thiocyanate (SCN-) degrading organisms that were isolated from electroplating wastewater and synthetic SCN- containing wastewater. PCR amplification of the 16S rRNA V1-V3 regions was undertaken using the 27F and 518R oligonucleotide primers following the metacommunity DNA extraction procedure. The PCR amplicons were processed using the illumina® reaction kits as per manufacturer׳s instruction and sequenced using the illumina® MiSeq-2000, using the MiSeq V3 kit. The data was processed using bioinformatics tools such as QIIME and the raw sequence files are available via NCBI׳s Sequence Read Archive (SRA) database.

KW - 16S rRNA gene

KW - Cyanide degrading organisms

KW - Metagenomics

KW - Thiocyanate degrading organisms

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U2 - 10.1016/j.dib.2017.06.049

DO - 10.1016/j.dib.2017.06.049

M3 - Article

AN - SCOPUS:85023636410

SN - 2352-3409

VL - 13

SP - 738

EP - 741

JO - Data in Brief

JF - Data in Brief

ER -

Metagenomic data of free cyanide and thiocyanate degrading bacterial communities

Abstract

Keywords

ASJC Scopus subject areas

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