Mechanism and antibacterial synergies of poly(Dabco-BBAC) nanoparticles against multi-drug resistant Pseudomonas aeruginosa isolates from human burns

Arefeh Ebadati, Mojgan Oshaghi, Sara Saeedi, Parastoo Parsa, Vahid Pirhajati Mahabadi, Morteza Karimi, Atefeh Jahandideh Hajiebrahimdehi, Michael R. Hamblin, Mahdi Karimi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Multi-drug resistant bacteria are a major problem in the treatment of infectious diseases, such as pneumonia, meningitis, or even coronavirus disease 2019 (COVID-19). Cationic nanopolymers are a new type of antimicrobial agent with high efficiency. We synthesized and characterized cationic polymer based on 1,4-diazabicyclo [2.2.2] octane (DABCO) and Bis (bromoacetyl)cystamine (BBAC), named poly (DABCO-BBAC) nanoparticles(NPs), and produced 150 nm diameter NPs. The antibacterial activity of poly (DABCO-BBAC) against eight multi drug resistant (MDR) Pseudomonas aeruginosa isolates from human burns, its possible synergistic effect with gentamicin, and the mechanism of action were examined. Poly(DABCO-BBAC) could effectively inhibit and kill bacterial strains at a very low concentration calculated by minimum inhibitory concentration (MIC) assay. Nevertheless, its synergism index with gentamicin showed an indifferent effect. Moreover, transmission electron microscopy and lipid peroxidation assays showed that poly (DABCO-BBAC) distorted and damaged the bacterial cell wall. These results suggest that the poly (DABCO-BBAC) could be an effective antibacterial agent for MDR clinical pathogens.

Original languageEnglish
Article number106718
JournalBioorganic Chemistry
Volume140
DOIs
Publication statusPublished - Nov 2023

Keywords

  • Antibacterial mechanism
  • Antibiotic synergism
  • Cationic nanopolymer
  • Multi-drug resistance, Poly(DABCO_BBAC) nanoparticles
  • Nanomedicine
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Organic Chemistry

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