TY - JOUR
T1 - Mechanism and antibacterial synergies of poly(Dabco-BBAC) nanoparticles against multi-drug resistant Pseudomonas aeruginosa isolates from human burns
AU - Ebadati, Arefeh
AU - Oshaghi, Mojgan
AU - Saeedi, Sara
AU - Parsa, Parastoo
AU - Mahabadi, Vahid Pirhajati
AU - Karimi, Morteza
AU - Hajiebrahimdehi, Atefeh Jahandideh
AU - Hamblin, Michael R.
AU - Karimi, Mahdi
N1 - Publisher Copyright:
© 2023
PY - 2023/11
Y1 - 2023/11
N2 - Multi-drug resistant bacteria are a major problem in the treatment of infectious diseases, such as pneumonia, meningitis, or even coronavirus disease 2019 (COVID-19). Cationic nanopolymers are a new type of antimicrobial agent with high efficiency. We synthesized and characterized cationic polymer based on 1,4-diazabicyclo [2.2.2] octane (DABCO) and Bis (bromoacetyl)cystamine (BBAC), named poly (DABCO-BBAC) nanoparticles(NPs), and produced 150 nm diameter NPs. The antibacterial activity of poly (DABCO-BBAC) against eight multi drug resistant (MDR) Pseudomonas aeruginosa isolates from human burns, its possible synergistic effect with gentamicin, and the mechanism of action were examined. Poly(DABCO-BBAC) could effectively inhibit and kill bacterial strains at a very low concentration calculated by minimum inhibitory concentration (MIC) assay. Nevertheless, its synergism index with gentamicin showed an indifferent effect. Moreover, transmission electron microscopy and lipid peroxidation assays showed that poly (DABCO-BBAC) distorted and damaged the bacterial cell wall. These results suggest that the poly (DABCO-BBAC) could be an effective antibacterial agent for MDR clinical pathogens.
AB - Multi-drug resistant bacteria are a major problem in the treatment of infectious diseases, such as pneumonia, meningitis, or even coronavirus disease 2019 (COVID-19). Cationic nanopolymers are a new type of antimicrobial agent with high efficiency. We synthesized and characterized cationic polymer based on 1,4-diazabicyclo [2.2.2] octane (DABCO) and Bis (bromoacetyl)cystamine (BBAC), named poly (DABCO-BBAC) nanoparticles(NPs), and produced 150 nm diameter NPs. The antibacterial activity of poly (DABCO-BBAC) against eight multi drug resistant (MDR) Pseudomonas aeruginosa isolates from human burns, its possible synergistic effect with gentamicin, and the mechanism of action were examined. Poly(DABCO-BBAC) could effectively inhibit and kill bacterial strains at a very low concentration calculated by minimum inhibitory concentration (MIC) assay. Nevertheless, its synergism index with gentamicin showed an indifferent effect. Moreover, transmission electron microscopy and lipid peroxidation assays showed that poly (DABCO-BBAC) distorted and damaged the bacterial cell wall. These results suggest that the poly (DABCO-BBAC) could be an effective antibacterial agent for MDR clinical pathogens.
KW - Antibacterial mechanism
KW - Antibiotic synergism
KW - Cationic nanopolymer
KW - Multi-drug resistance, Poly(DABCO_BBAC) nanoparticles
KW - Nanomedicine
KW - Pseudomonas aeruginosa
UR - http://www.scopus.com/inward/record.url?scp=85167438187&partnerID=8YFLogxK
U2 - 10.1016/j.bioorg.2023.106718
DO - 10.1016/j.bioorg.2023.106718
M3 - Article
C2 - 37566942
AN - SCOPUS:85167438187
SN - 0045-2068
VL - 140
JO - Bioorganic Chemistry
JF - Bioorganic Chemistry
M1 - 106718
ER -
