Low-level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair

Felipe F. Sperandio; Alyne Simões; Luciana Corrêa; Ana Cecília C. Aranha; Fernanda S. Giudice; Michael R. Hamblin; Suzana C.O.M. Sousa

doi:10.1002/jbio.201400064

Low-level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair

Felipe F. Sperandio, Alyne Simões, Luciana Corrêa, Ana Cecília C. Aranha, Fernanda S. Giudice, Michael R. Hamblin, Suzana C.O.M. Sousa

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

Low-level laser therapy (LLLT) has been extensively employed to improve epithelial wound healing, though the exact response of epithelium maturation and stratification after LLLT is unknown. Thus, this study aimed to assess the in vitro growth and differentiation of keratinocytes (KCs) and in vivo wound healing response when treated with LLLT. Human KCs (HaCaT cells) showed an enhanced proliferation with all the employed laser energy densities (3, 6 and 12 J/cm², 660 nm, 100 mW), together with an increased expression of Cyclin D1. Moreover, the immunoexpression of proteins related to epithelial proliferation and maturation (p63, CK10, CK14) all indicated a faster maturation of the migrating KCs in the LLLT-treated wounds. In that way, an improved epithelial healing was promoted by LLLT with the employed parameters; this improvement was confirmed by changes in the expression of several proteins related to epithelial proliferation and maturation.

Original language	English
Pages (from-to)	795-803
Number of pages	9
Journal	Journal of Biophotonics
Volume	8
Issue number	10
DOIs	https://doi.org/10.1002/jbio.201400064
Publication status	Published - Oct 2015
Externally published	Yes

Keywords

Cyclin D1
HaCaT keratinocytes
Keratins
Low-level laser therapy
P63 protein
Photobiomodulation
Wound healing

ASJC Scopus subject areas

General Chemistry
General Materials Science
General Biochemistry,Genetics and Molecular Biology
General Engineering
General Physics and Astronomy

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10.1002/jbio.201400064

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title = "Low-level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair",

abstract = "Low-level laser therapy (LLLT) has been extensively employed to improve epithelial wound healing, though the exact response of epithelium maturation and stratification after LLLT is unknown. Thus, this study aimed to assess the in vitro growth and differentiation of keratinocytes (KCs) and in vivo wound healing response when treated with LLLT. Human KCs (HaCaT cells) showed an enhanced proliferation with all the employed laser energy densities (3, 6 and 12 J/cm2, 660 nm, 100 mW), together with an increased expression of Cyclin D1. Moreover, the immunoexpression of proteins related to epithelial proliferation and maturation (p63, CK10, CK14) all indicated a faster maturation of the migrating KCs in the LLLT-treated wounds. In that way, an improved epithelial healing was promoted by LLLT with the employed parameters; this improvement was confirmed by changes in the expression of several proteins related to epithelial proliferation and maturation.",

keywords = "Cyclin D1, HaCaT keratinocytes, Keratins, Low-level laser therapy, P63 protein, Photobiomodulation, Wound healing",

author = "Sperandio, {Felipe F.} and Alyne Sim{\~o}es and Luciana Corr{\^e}a and Aranha, {Ana Cec{\'i}lia C.} and Giudice, {Fernanda S.} and Hamblin, {Michael R.} and Sousa, {Suzana C.O.M.}",

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year = "2015",

month = oct,

doi = "10.1002/jbio.201400064",

language = "English",

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T1 - Low-level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair

AU - Sperandio, Felipe F.

AU - Simões, Alyne

AU - Corrêa, Luciana

AU - Aranha, Ana Cecília C.

AU - Giudice, Fernanda S.

AU - Hamblin, Michael R.

AU - Sousa, Suzana C.O.M.

PY - 2015/10

Y1 - 2015/10

N2 - Low-level laser therapy (LLLT) has been extensively employed to improve epithelial wound healing, though the exact response of epithelium maturation and stratification after LLLT is unknown. Thus, this study aimed to assess the in vitro growth and differentiation of keratinocytes (KCs) and in vivo wound healing response when treated with LLLT. Human KCs (HaCaT cells) showed an enhanced proliferation with all the employed laser energy densities (3, 6 and 12 J/cm2, 660 nm, 100 mW), together with an increased expression of Cyclin D1. Moreover, the immunoexpression of proteins related to epithelial proliferation and maturation (p63, CK10, CK14) all indicated a faster maturation of the migrating KCs in the LLLT-treated wounds. In that way, an improved epithelial healing was promoted by LLLT with the employed parameters; this improvement was confirmed by changes in the expression of several proteins related to epithelial proliferation and maturation.

AB - Low-level laser therapy (LLLT) has been extensively employed to improve epithelial wound healing, though the exact response of epithelium maturation and stratification after LLLT is unknown. Thus, this study aimed to assess the in vitro growth and differentiation of keratinocytes (KCs) and in vivo wound healing response when treated with LLLT. Human KCs (HaCaT cells) showed an enhanced proliferation with all the employed laser energy densities (3, 6 and 12 J/cm2, 660 nm, 100 mW), together with an increased expression of Cyclin D1. Moreover, the immunoexpression of proteins related to epithelial proliferation and maturation (p63, CK10, CK14) all indicated a faster maturation of the migrating KCs in the LLLT-treated wounds. In that way, an improved epithelial healing was promoted by LLLT with the employed parameters; this improvement was confirmed by changes in the expression of several proteins related to epithelial proliferation and maturation.

KW - Cyclin D1

KW - HaCaT keratinocytes

KW - Keratins

KW - Low-level laser therapy

KW - P63 protein

KW - Photobiomodulation

KW - Wound healing

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AN - SCOPUS:84944578573

SN - 1864-063X

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JO - Journal of Biophotonics

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IS - 10

ER -

Low-level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair

Abstract

Keywords

ASJC Scopus subject areas

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