Irradiation at 660 nm modulates different genes central to wound healing in wounded and diabetic wounded cell models

Nicolette N. Houreld

doi:10.1117/12.2041430

Irradiation at 660 nm modulates different genes central to wound healing in wounded and diabetic wounded cell models

Nicolette N. Houreld

Laser Research Centre

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Citation (Scopus)

Abstract

Wound healing is a highly orchestrated process and involves a wide variety of cellular components, chemokines and growth factors. Laser irradiation has influenced gene expression and release of various growth factors, cytokines and extracellular matrix proteins involved in wound healing. This study aimed to determine the expression profile of genes involved in wound healing in wounded and diabetic wounded fibroblast cells in response to irradiation at a wavelength of 660 nm. Human skin fibroblast cells (WS1) were irradiated with a diode laser (wavelength 660 nm; fluence 5 J/cm²; power output 100 mW; power density 11 mW/cm²; spot size 9.1 cm²; exposure duration 7 min 35 s). Total RNA was isolated and 1 μg reverse transcribed into cDNA which was used as a template in real-time qualitative polymerase chain reaction (qPCR). Eighty four genes involved in wound healing (extracellular matrix and cell adhesion; inflammatory cytokines and chemokines; growth factors; and signal transduction) were evaluated in wounded and diabetic wounded cell models. Forty eight hours post-irradiation, 6 genes were significantly upregulated and 8 genes were down-regulated in irradiated wounded cells, whereas 1 gene was up-regulated and 33 genes down-regulated in irradiated diabetic wounded cells. Irradiation of stressed fibroblast cells to a wavelength of 660 nm and a fluence of 5 J/cm² modulated the expression of different genes involved in wound healing in different cell models. Modulation of these genes leads to the effects of laser irradiation seen both in vivo and in vitro, and facilitates the wound healing process.

Original language	English
Title of host publication	Mechanisms for Low-Light Therapy IX
Publisher	SPIE
ISBN (Print)	9780819498458
DOIs	https://doi.org/10.1117/12.2041430
Publication status	Published - 2014
Event	Mechanisms for Low-Light Therapy IX - San Francisco, CA, United States Duration: 1 Feb 2014 → 2 Feb 2014

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	8932
ISSN (Print)	1605-7422

Conference

Conference	Mechanisms for Low-Light Therapy IX
Country/Territory	United States
City	San Francisco, CA
Period	1/02/14 → 2/02/14

Keywords

Diabetes
Extracellular matrix
Gene profile
LLLT
Laser irradiation
QPCR
Wound healing

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology, Nuclear Medicine and Imaging

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1117/12.2041430

Cite this

@inproceedings{2cc1eab0967f4606af0eb697eb98bc60,

title = "Irradiation at 660 nm modulates different genes central to wound healing in wounded and diabetic wounded cell models",

abstract = "Wound healing is a highly orchestrated process and involves a wide variety of cellular components, chemokines and growth factors. Laser irradiation has influenced gene expression and release of various growth factors, cytokines and extracellular matrix proteins involved in wound healing. This study aimed to determine the expression profile of genes involved in wound healing in wounded and diabetic wounded fibroblast cells in response to irradiation at a wavelength of 660 nm. Human skin fibroblast cells (WS1) were irradiated with a diode laser (wavelength 660 nm; fluence 5 J/cm2; power output 100 mW; power density 11 mW/cm2; spot size 9.1 cm2; exposure duration 7 min 35 s). Total RNA was isolated and 1 μg reverse transcribed into cDNA which was used as a template in real-time qualitative polymerase chain reaction (qPCR). Eighty four genes involved in wound healing (extracellular matrix and cell adhesion; inflammatory cytokines and chemokines; growth factors; and signal transduction) were evaluated in wounded and diabetic wounded cell models. Forty eight hours post-irradiation, 6 genes were significantly upregulated and 8 genes were down-regulated in irradiated wounded cells, whereas 1 gene was up-regulated and 33 genes down-regulated in irradiated diabetic wounded cells. Irradiation of stressed fibroblast cells to a wavelength of 660 nm and a fluence of 5 J/cm2 modulated the expression of different genes involved in wound healing in different cell models. Modulation of these genes leads to the effects of laser irradiation seen both in vivo and in vitro, and facilitates the wound healing process.",

keywords = "Diabetes, Extracellular matrix, Gene profile, LLLT, Laser irradiation, QPCR, Wound healing",

author = "Houreld, {Nicolette N.}",

year = "2014",

doi = "10.1117/12.2041430",

language = "English",

isbn = "9780819498458",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

booktitle = "Mechanisms for Low-Light Therapy IX",

address = "United States",

note = "Mechanisms for Low-Light Therapy IX ; Conference date: 01-02-2014 Through 02-02-2014",

}

Houreld, NN 2014, Irradiation at 660 nm modulates different genes central to wound healing in wounded and diabetic wounded cell models. in Mechanisms for Low-Light Therapy IX., 89320A, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8932, SPIE, Mechanisms for Low-Light Therapy IX, San Francisco, CA, United States, 1/02/14. https://doi.org/10.1117/12.2041430

Irradiation at 660 nm modulates different genes central to wound healing in wounded and diabetic wounded cell models. / Houreld, Nicolette N.
Mechanisms for Low-Light Therapy IX. SPIE, 2014. 89320A (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8932).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Irradiation at 660 nm modulates different genes central to wound healing in wounded and diabetic wounded cell models

AU - Houreld, Nicolette N.

PY - 2014

Y1 - 2014

N2 - Wound healing is a highly orchestrated process and involves a wide variety of cellular components, chemokines and growth factors. Laser irradiation has influenced gene expression and release of various growth factors, cytokines and extracellular matrix proteins involved in wound healing. This study aimed to determine the expression profile of genes involved in wound healing in wounded and diabetic wounded fibroblast cells in response to irradiation at a wavelength of 660 nm. Human skin fibroblast cells (WS1) were irradiated with a diode laser (wavelength 660 nm; fluence 5 J/cm2; power output 100 mW; power density 11 mW/cm2; spot size 9.1 cm2; exposure duration 7 min 35 s). Total RNA was isolated and 1 μg reverse transcribed into cDNA which was used as a template in real-time qualitative polymerase chain reaction (qPCR). Eighty four genes involved in wound healing (extracellular matrix and cell adhesion; inflammatory cytokines and chemokines; growth factors; and signal transduction) were evaluated in wounded and diabetic wounded cell models. Forty eight hours post-irradiation, 6 genes were significantly upregulated and 8 genes were down-regulated in irradiated wounded cells, whereas 1 gene was up-regulated and 33 genes down-regulated in irradiated diabetic wounded cells. Irradiation of stressed fibroblast cells to a wavelength of 660 nm and a fluence of 5 J/cm2 modulated the expression of different genes involved in wound healing in different cell models. Modulation of these genes leads to the effects of laser irradiation seen both in vivo and in vitro, and facilitates the wound healing process.

AB - Wound healing is a highly orchestrated process and involves a wide variety of cellular components, chemokines and growth factors. Laser irradiation has influenced gene expression and release of various growth factors, cytokines and extracellular matrix proteins involved in wound healing. This study aimed to determine the expression profile of genes involved in wound healing in wounded and diabetic wounded fibroblast cells in response to irradiation at a wavelength of 660 nm. Human skin fibroblast cells (WS1) were irradiated with a diode laser (wavelength 660 nm; fluence 5 J/cm2; power output 100 mW; power density 11 mW/cm2; spot size 9.1 cm2; exposure duration 7 min 35 s). Total RNA was isolated and 1 μg reverse transcribed into cDNA which was used as a template in real-time qualitative polymerase chain reaction (qPCR). Eighty four genes involved in wound healing (extracellular matrix and cell adhesion; inflammatory cytokines and chemokines; growth factors; and signal transduction) were evaluated in wounded and diabetic wounded cell models. Forty eight hours post-irradiation, 6 genes were significantly upregulated and 8 genes were down-regulated in irradiated wounded cells, whereas 1 gene was up-regulated and 33 genes down-regulated in irradiated diabetic wounded cells. Irradiation of stressed fibroblast cells to a wavelength of 660 nm and a fluence of 5 J/cm2 modulated the expression of different genes involved in wound healing in different cell models. Modulation of these genes leads to the effects of laser irradiation seen both in vivo and in vitro, and facilitates the wound healing process.

KW - Diabetes

KW - Extracellular matrix

KW - Gene profile

KW - LLLT

KW - Laser irradiation

KW - QPCR

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=84896963260&partnerID=8YFLogxK

U2 - 10.1117/12.2041430

DO - 10.1117/12.2041430

M3 - Conference contribution

AN - SCOPUS:84896963260

SN - 9780819498458

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Mechanisms for Low-Light Therapy IX

PB - SPIE

T2 - Mechanisms for Low-Light Therapy IX

Y2 - 1 February 2014 through 2 February 2014

ER -

Irradiation at 660 nm modulates different genes central to wound healing in wounded and diabetic wounded cell models

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this