Investigation of the metabolic profile and toxigenic variability of fungal species occurring in fermented foods and beverage from nigeria and South Africa using UPLC-MS/MS

Ifeoluwa Adekoya, Patrick Njobeh, Adewale Obadina, Sofie Landschoot, Kris Audenaert, Sheila Okoth, Marthe De Boevre, Sarah De Saeger

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Fungal species recovered from fermented foods and beverage from Nigeria and South Africa were studied to establish their toxigenic potential in producing an array of secondary metabolites including mycotoxins (n = 49) that could compromise human and animal safety. In total, 385 fungal isolates were grown on solidified yeast extract sucrose agar. Their metabolites were extracted and analyzed via ultra-performance liquid chromatography tandem mass spectrometry. To examine the grouping of isolates and co-occurrence of metabolites, hierarchal clustering and pairwise association analysis was performed. Of the 385 fungal strains tested, over 41% were toxigenic producing different mycotoxins. A. flavus and A. parasiticus strains were the principal producers of aflatoxin B 1 (27-7406 µg/kg). Aflatoxin B 1 and cyclopiazonic acid had a positive association. Ochratoxin A was produced by 67% of the A. niger strains in the range of 28-1302 µg/kg. The sterigmatocystin producers found were A. versicolor (n = 12), A. amstelodami (n = 4), and A. sydowii (n = 6). Apart from P. chrysogenum, none of the Penicillium spp. produced roquefortine C. Amongst the Fusarium strains tested, F. verticillioides produced fumonisin B 1 (range: 77-218 µg/kg) meanwhile low levels of deoxynivalenol were observed. The production of multiple metabolites by single fungal species was also evident.

Original languageEnglish
Article number85
JournalToxins
Volume11
Issue number2
DOIs
Publication statusPublished - Feb 2019

Keywords

  • Aspergillus
  • Fermented foods
  • Metabolites
  • Mycotoxins
  • Toxigenicity
  • UPLC-MS/MS

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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