Injection of Cerium Oxide Nanoparticles to Treat Spinal Cord Injury in Rats

Zahra Behroozi, Behnaz Rahimi, Michael R. Hamblin, Farinaz Nasirinezhad, Atousa Janzadeh, Fatemeh Ramezani

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

This study investigated the effects of local injection of cerium oxide nanoparticles (CeONPs) in a rat spinal cord injury (SCI) model. Thirty-six adult male Wistar rats were divided into 4 groups: controls (healthy animals), sham (laminectomy), SCI (laminectomyþSCI induction), and treatment (laminectomyþSCI inductionþintrathecal injection of CeONPs immediately after injury). SCI was induced using an aneurysm clip at the T12-T13 vertebral region. Motor performance and pain threshold tests were performed weekly; H&E staining and measurement of cavity sizes were performed 6 weeks after injury. The expression of granulocyte colony-stimulating factor (GCSF), P44/42 MAPK, P-P44/42 MAPK, Tau, myelin-associated glycoprotein(MAG) was evaluated after 6 weeks by Western blot. The Basso, Beattie, and Bresnahan locomotor scoring scales improved in animals receiving CeONPs compared with SCI animals. The cavity sizes were less in the treatment group. GCSF expression was similar in the animals receiving CeONPs compared with the SCI group but the expression of ERK1/ ERK2 and phospho-ERK was lower than in the SCI group. Expression levels of Tau and MAG were significantly increased in treated animals compared to the SCI group. These data indicate that the use of CeONPs may improve motor functional recovery in SCI.

Original languageEnglish
Pages (from-to)635-642
Number of pages8
JournalJournal of Neuropathology and Experimental Neurology
Volume81
Issue number8
DOIs
Publication statusPublished - 1 Aug 2022

Keywords

  • Cerium oxide nanoparticles
  • Neural cells
  • Regeneration
  • Spinal cord injury

ASJC Scopus subject areas

  • General Medicine

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