Influence of nitro ruthenium isomerization on photochemically induced nitric oxide release: Vasorelaxant activities

Alexia Marques Silva; Laísa Bonafim Negri; Juliana Cristina Biazzotto; Sergio de Paula Machado; Jeimison Duarte Santos; Jorge Fernandes Nasser Batista; Pedro Ivo S. Maia; Victor Marcelo Deflon; Lusiane M. Bendhack; Michael R. Hamblin; Roberto S. da Silva

doi:10.1016/j.jinorgbio.2023.112166

Influence of nitro ruthenium isomerization on photochemically induced nitric oxide release: Vasorelaxant activities

Alexia Marques Silva
, Laísa Bonafim Negri
, Juliana Cristina Biazzotto
, Sergio de Paula Machado
, Jeimison Duarte Santos
, Jorge Fernandes Nasser Batista
, Pedro Ivo S. Maia
, Victor Marcelo Deflon
, Lusiane M. Bendhack
, Michael R. Hamblin
, Roberto S. da Silva

Laser Research Centre

Universidade de São Paulo
Ohio State University
Massachusetts General Hospital
Harvard University
Universidade Federal do Rio de Janeiro
Universidade Federal do Triângulo Mineiro

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We have synthesized cis-[Ru(bpy)₂(NO₂-κN)L^n-]^(n-1) and cis-[Ru(bpy)₂(NO₂-κO)L ^n-]^(n-1) (bpy = 2,2′-bipyridine; k = indication of the coordinated center to Ruthenium; L = pyridine type ligand) by reacting cis-[Ru(bpy)₂(H₂O)L^n-]^(n-2) with sodium nitrite or conducting basic cis-[Ru(bpy)₂NO(L^n-)]^(n-3) hydrolysis. Photolysis at the metal-ligand charge transfer band (MLCT) of the isomers yielded nitric oxide (NO) as determined by NO measurement. The NO photorelease rates obtained upon 447 nm laser irradiation of the ruthenium complexes showed that cis-[Ru(bpy)₂(NO₂-κO)L^n-]^(n-1) released NO three times faster than cis-[Ru(bpy)₂(NO₂-κN)L^n-]^(n-1). We investigated endothelium-dependent vasodilation induced by cis-[Ru(bpy)₂(4-pic)(NO₂-κN)]⁺ and cis-[Ru(bpy)₂(4-pic)(NO₂-κO)]⁺ (4-pic = 4-picoline) in isolated 3 mm aortic rings precontracted with L-phenylephrine. Maximum vasodilation was achieved under 447 nm laser irradiation of 0.5 μMol.L⁻¹ ruthenium complexes for 100 s.

Original language	English
Article number	112166
Journal	Journal of Inorganic Biochemistry
Volume	243
DOIs	https://doi.org/10.1016/j.jinorgbio.2023.112166
Publication status	Published - Jun 2023

Keywords

Metal complexes
Nitric oxide
Nitro ruthenium (II)
Nitro ruthenium-bipyridine
Photorelease
Vasodilation

ASJC Scopus subject areas

Biochemistry
Inorganic Chemistry

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10.1016/j.jinorgbio.2023.112166

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Silva, A. M., Negri, L. B., Biazzotto, J. C., de Paula Machado, S., Santos, J. D., Batista, J. F. N., Maia, P. I. S., Deflon, V. M., Bendhack, L. M., Hamblin, M. R., & da Silva, R. S. (2023). Influence of nitro ruthenium isomerization on photochemically induced nitric oxide release: Vasorelaxant activities. Journal of Inorganic Biochemistry, 243, Article 112166. https://doi.org/10.1016/j.jinorgbio.2023.112166

@article{5aa6d1d961814dd79548a246c346044f,

title = "Influence of nitro ruthenium isomerization on photochemically induced nitric oxide release: Vasorelaxant activities",

abstract = "We have synthesized cis-[Ru(bpy)2(NO2-κN)Ln-](n-1) and cis-[Ru(bpy)2(NO2-κO)L n-](n-1) (bpy = 2,2′-bipyridine; k = indication of the coordinated center to Ruthenium; L = pyridine type ligand) by reacting cis-[Ru(bpy)2(H2O)Ln-](n-2) with sodium nitrite or conducting basic cis-[Ru(bpy)2NO(Ln-)](n-3) hydrolysis. Photolysis at the metal-ligand charge transfer band (MLCT) of the isomers yielded nitric oxide (NO) as determined by NO measurement. The NO photorelease rates obtained upon 447 nm laser irradiation of the ruthenium complexes showed that cis-[Ru(bpy)2(NO2-κO)Ln-](n-1) released NO three times faster than cis-[Ru(bpy)2(NO2-κN)Ln-](n-1). We investigated endothelium-dependent vasodilation induced by cis-[Ru(bpy)2(4-pic)(NO2-κN)]+ and cis-[Ru(bpy)2(4-pic)(NO2-κO)]+ (4-pic = 4-picoline) in isolated 3 mm aortic rings precontracted with L-phenylephrine. Maximum vasodilation was achieved under 447 nm laser irradiation of 0.5 μMol.L−1 ruthenium complexes for 100 s.",

keywords = "Metal complexes, Nitric oxide, Nitro ruthenium (II), Nitro ruthenium-bipyridine, Photorelease, Vasodilation",

author = "Silva, \{Alexia Marques\} and Negri, \{La{\'i}sa Bonafim\} and Biazzotto, \{Juliana Cristina\} and \{de Paula Machado\}, Sergio and Santos, \{Jeimison Duarte\} and Batista, \{Jorge Fernandes Nasser\} and Maia, \{Pedro Ivo S.\} and Deflon, \{Victor Marcelo\} and Bendhack, \{Lusiane M.\} and Hamblin, \{Michael R.\} and \{da Silva\}, \{Roberto S.\}",

note = "Publisher Copyright: {\textcopyright} 2023",

year = "2023",

month = jun,

doi = "10.1016/j.jinorgbio.2023.112166",

language = "English",

volume = "243",

journal = "Journal of Inorganic Biochemistry",

issn = "0162-0134",

publisher = "Elsevier Inc.",

}

Silva, AM, Negri, LB, Biazzotto, JC, de Paula Machado, S, Santos, JD, Batista, JFN, Maia, PIS, Deflon, VM, Bendhack, LM, Hamblin, MR & da Silva, RS 2023, 'Influence of nitro ruthenium isomerization on photochemically induced nitric oxide release: Vasorelaxant activities', Journal of Inorganic Biochemistry, vol. 243, 112166. https://doi.org/10.1016/j.jinorgbio.2023.112166

TY - JOUR

T1 - Influence of nitro ruthenium isomerization on photochemically induced nitric oxide release

T2 - Vasorelaxant activities

AU - Silva, Alexia Marques

AU - Negri, Laísa Bonafim

AU - Biazzotto, Juliana Cristina

AU - de Paula Machado, Sergio

AU - Santos, Jeimison Duarte

AU - Batista, Jorge Fernandes Nasser

AU - Maia, Pedro Ivo S.

AU - Deflon, Victor Marcelo

AU - Bendhack, Lusiane M.

AU - Hamblin, Michael R.

AU - da Silva, Roberto S.

PY - 2023/6

Y1 - 2023/6

N2 - We have synthesized cis-[Ru(bpy)2(NO2-κN)Ln-](n-1) and cis-[Ru(bpy)2(NO2-κO)L n-](n-1) (bpy = 2,2′-bipyridine; k = indication of the coordinated center to Ruthenium; L = pyridine type ligand) by reacting cis-[Ru(bpy)2(H2O)Ln-](n-2) with sodium nitrite or conducting basic cis-[Ru(bpy)2NO(Ln-)](n-3) hydrolysis. Photolysis at the metal-ligand charge transfer band (MLCT) of the isomers yielded nitric oxide (NO) as determined by NO measurement. The NO photorelease rates obtained upon 447 nm laser irradiation of the ruthenium complexes showed that cis-[Ru(bpy)2(NO2-κO)Ln-](n-1) released NO three times faster than cis-[Ru(bpy)2(NO2-κN)Ln-](n-1). We investigated endothelium-dependent vasodilation induced by cis-[Ru(bpy)2(4-pic)(NO2-κN)]+ and cis-[Ru(bpy)2(4-pic)(NO2-κO)]+ (4-pic = 4-picoline) in isolated 3 mm aortic rings precontracted with L-phenylephrine. Maximum vasodilation was achieved under 447 nm laser irradiation of 0.5 μMol.L−1 ruthenium complexes for 100 s.

AB - We have synthesized cis-[Ru(bpy)2(NO2-κN)Ln-](n-1) and cis-[Ru(bpy)2(NO2-κO)L n-](n-1) (bpy = 2,2′-bipyridine; k = indication of the coordinated center to Ruthenium; L = pyridine type ligand) by reacting cis-[Ru(bpy)2(H2O)Ln-](n-2) with sodium nitrite or conducting basic cis-[Ru(bpy)2NO(Ln-)](n-3) hydrolysis. Photolysis at the metal-ligand charge transfer band (MLCT) of the isomers yielded nitric oxide (NO) as determined by NO measurement. The NO photorelease rates obtained upon 447 nm laser irradiation of the ruthenium complexes showed that cis-[Ru(bpy)2(NO2-κO)Ln-](n-1) released NO three times faster than cis-[Ru(bpy)2(NO2-κN)Ln-](n-1). We investigated endothelium-dependent vasodilation induced by cis-[Ru(bpy)2(4-pic)(NO2-κN)]+ and cis-[Ru(bpy)2(4-pic)(NO2-κO)]+ (4-pic = 4-picoline) in isolated 3 mm aortic rings precontracted with L-phenylephrine. Maximum vasodilation was achieved under 447 nm laser irradiation of 0.5 μMol.L−1 ruthenium complexes for 100 s.

KW - Metal complexes

KW - Nitric oxide

KW - Nitro ruthenium (II)

KW - Nitro ruthenium-bipyridine

KW - Photorelease

KW - Vasodilation

UR - https://www.scopus.com/pages/publications/85151880607

U2 - 10.1016/j.jinorgbio.2023.112166

DO - 10.1016/j.jinorgbio.2023.112166

M3 - Article

C2 - 36947899

AN - SCOPUS:85151880607

SN - 0162-0134

VL - 243

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

M1 - 112166

ER -

Influence of nitro ruthenium isomerization on photochemically induced nitric oxide release: Vasorelaxant activities

Abstract

Keywords

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