Influence of bacterial interactions on the susceptibility to photodynamic inactivation

M. H. Upadya, G. Tegos, M. Hamblin, A. Kishen

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Photodynamic therapy has emerged as a possible supplement to the existing protocols for endodontic disinfection. Microbes are known to gain significant ecological advantage when they survive as coaggregates and biofilms in an infected tissue. Such microbial coaggregates and biofilms have been confirmed to play a key role in the pathogenicity of many infections. So far, not many studies have correlated the efficacy of antimicrobial photodynamic inactivation (APDI) to the different modes of bacterial growth. This study aims to evaluate the APDI of 3 strains of Enterococcus faecalis in planktonic phase, in a co-aggregated suspension and in a 4-day old biofilm. The results showed that the biofilm mode of growth offered the greatest resistance to APDI and the inclusion of an efflux pump inhibitor significantly increased the APDI of biofilm bacteria. From this study, we conclude that APDI of bacteria in biofilms is the most challenging and that the use of bacterial efflux pump inhibitors enhances its photodynamic antibiofilm efficacy.

Original languageEnglish
Title of host publication12th World Congress of the International Photodynamic Association - Photodynamic Therapy
Subtitle of host publicationBack to the Future
DOIs
Publication statusPublished - 2009
Externally publishedYes
Event12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future - Seattle, WA, United States
Duration: 11 Jun 200915 Jun 2009

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7380
ISSN (Print)1605-7422

Conference

Conference12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future
Country/TerritoryUnited States
CitySeattle, WA
Period11/06/0915/06/09

Keywords

  • Enterococcus faecalis
  • biofilm
  • efflux pump inhibitor
  • photodynamic therapy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology, Nuclear Medicine and Imaging

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