In Vivo investigation of antimicrobial blue light therapy for multidrug-resistant Acinetobacter baumannii burn infections using bioluminescence imaging

Yucheng Wang, Olivia D. Harrington, Ying Wang, Clinton K. Murray, Michael R. Hamblin, Tianhong Dai

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Burn infections continue to be an important cause of morbidity and mortality. The increasing emergence of multidrug-resistant (MDR) bacteria has led to the frequent failure of traditional antibiotic treatments. Alternative therapeutics are urgently needed to tackle MDR bacteria. An innovative non-antibiotic approach, antimicrobial blue light (aBL), has shown promising effectiveness against MDR infections. The mechanism of action of aBL is not yet well understood. It is commonly hypothesized that naturally occurring endogenous photosensitizing chromophores in bacteria (e.g., iron-free porphyrins, flavins, etc.) are excited by aBL, which in turn produces cytotoxic reactive oxygen species (ROS) through a photochemical process. Unlike another light-based antimicrobial approach, antimicrobial photodynamic therapy (aPDT), aBL therapy does not require the involvement of an exogenous photosensitizer. All it needs to take effect is the irradiation of blue light; therefore, it is simple and inexpensive. The aBL receptors are the endogenous cellular photosensitizers in bacteria, rather than the DNA. Thus, aBL is believed to be much less genotoxic to host cells than ultraviolet-C (UVC) irradiation, which directly causes DNA damage in host cells. In this paper, we present a protocol to assess the effectiveness of aBL therapy for MDR Acinetobacter baumannii infections in a mouse model of burn injury. By using an engineered bioluminescent strain, we were able to noninvasively monitor the extent of infection in real time in living animals. This technique is also an effective tool for monitoring the spatial distribution of infections in animals.

Original languageEnglish
Article numbere54997
JournalJournal of Visualized Experiments
Volume2017
Issue number122
DOIs
Publication statusPublished - 28 Apr 2017
Externally publishedYes

Keywords

  • Acinetobacter baumannii
  • Antimicrobial blue light
  • Bioluminescence imaging
  • Burn
  • Immunology
  • Infection
  • Issue 122
  • Mouse model
  • Multidrug resistance

ASJC Scopus subject areas

  • General Neuroscience
  • General Chemical Engineering
  • General Biochemistry,Genetics and Molecular Biology
  • General Immunology and Microbiology

Fingerprint

Dive into the research topics of 'In Vivo investigation of antimicrobial blue light therapy for multidrug-resistant Acinetobacter baumannii burn infections using bioluminescence imaging'. Together they form a unique fingerprint.

Cite this