TY - JOUR
T1 - In vitro, ex vivo, and in silico studies of phytochemicals from the stem bark of Bischofia javanica
AU - Awas, Erick Okoth
AU - Midiwo, Jacob O.
AU - Omosa, Leonidah Kerubo
AU - Derese, Solomon
AU - Kiganda, Ivan
AU - Omole, Ruth A.
AU - Akala, Hosea M.
AU - Ochora, Douglas O.
AU - Obakachi, Vincent Adeiza
AU - Olalekan, Samuel
AU - Govender, Krishna Kuben
AU - Nchiozem-Ngnitedem, Vaderament A.
N1 - Publisher Copyright:
© 2025 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2025
Y1 - 2025
N2 - The stem bark extract of Bischofia javanica, used in traditional Kenyan medicine afforded nine compounds named, santin (1), ermanin (2), rhamnocitrin (3), kumatakenin (4), abyssinone V-4′-methyl ether (5), β-sitosterol (6), betulinic acid (7), friedelin (8) and 1-methyl-1,10-phenanthrolin-1-ium (9). Structural elucidation was performed based on spectroscopic and spectrometric analyses, and the data were compared with those reported in the literature. The natural origin of 1-methyl-1,10-phenanthrolin-1-ium (9) in the plant kingdom is reported here for the first time. Ermanin (2) showed selective in vitro antiplasmodial activity with IC50 = 0.05 μM, while 1-methyl-1,10-phenanthrolinium (9) exhibited potent antiplasmodial activity with IC50 values of 1.56 μM (in vitro), and 1.20 μM (ex vivo). Furthermore, molecular docking studies revealed that 1-methyl-1,10-phenanthrolinium (9) could be considered a potential lead compound for future antimalarial drugs.
AB - The stem bark extract of Bischofia javanica, used in traditional Kenyan medicine afforded nine compounds named, santin (1), ermanin (2), rhamnocitrin (3), kumatakenin (4), abyssinone V-4′-methyl ether (5), β-sitosterol (6), betulinic acid (7), friedelin (8) and 1-methyl-1,10-phenanthrolin-1-ium (9). Structural elucidation was performed based on spectroscopic and spectrometric analyses, and the data were compared with those reported in the literature. The natural origin of 1-methyl-1,10-phenanthrolin-1-ium (9) in the plant kingdom is reported here for the first time. Ermanin (2) showed selective in vitro antiplasmodial activity with IC50 = 0.05 μM, while 1-methyl-1,10-phenanthrolinium (9) exhibited potent antiplasmodial activity with IC50 values of 1.56 μM (in vitro), and 1.20 μM (ex vivo). Furthermore, molecular docking studies revealed that 1-methyl-1,10-phenanthrolinium (9) could be considered a potential lead compound for future antimalarial drugs.
KW - 1-methyl-1
KW - 10-phenanthrolin-1-ium
KW - Bischofia javanica
KW - Phyllanthaceae
KW - antiplasmodial
UR - https://www.scopus.com/pages/publications/105008288733
U2 - 10.1080/14786419.2025.2521380
DO - 10.1080/14786419.2025.2521380
M3 - Article
AN - SCOPUS:105008288733
SN - 1478-6419
JO - Natural Product Research
JF - Natural Product Research
ER -
