Improved sensitivity of tuberculosis antigen MPT64 detection using SPR phase difference

Tuberculosis (TB) remains a significant global health concern, necessitating the development of sensitive and efficient diagnostic methods. This study compares the performance of the custom-built Surface Plasmon Resonance (SPR) phase difference with conventional SPR in detecting the tuberculosis antigen MPT64. Our objective is to evaluate whether the SPR phase shift offers improved sensitivity compared to the conventional SPR technique. MPT64 antigen samples with varying concentrations were prepared and passed over the sensor chip. The resulting binding events were monitored using SPR phase difference and conventional SPR measurement techniques. Our findings demonstrate that SPR phase difference exhibits enhanced sensitivity compared to conventional SPR in the detection of MPT64 antigen. The SPR phase shift technique effectively amplifies the signal response and offers a greater dynamic range, enabling the detection of lower antigen concentrations. The experimental results were analyzed statistically, comparing the limits of detection between SPR phase difference and conventional SPR. The data indicate that the SPR phase difference outperforms the conventional SPR method in terms of sensitivity, highlighting its potential as a superior tool for TB antigen detection. The results obtained in this study may contribute to the advancement of TB diagnosis. The improved sensitivity offered by the SPR phase difference technique has promising implications for early and accurate TB diagnosis, aiding in the timely initiation of treatment and reducing disease transmission.