Identification of mamp-responsive plasma membrane-associated proteins in arabidopsis thaliana following challenge with different LPS chemotypes from xanthomonas campestris

Raeesa H. Hussan, Ian A. Dubery, Lizelle A. Piater

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Lipopolysaccharides (LPS) are recognized as microbe-associated molecular patterns (MAMPs) responsible for eliciting defense-related responses and while the effects have been well-documented in mammals, there is a lack of knowledge regarding the mechanism of perception in plant systems and recognized structural moieties within the macromolecular lipoglycan structure. Thus, identification of the LPS plasma membrane (PM) receptor(s)/receptor complex in Arabidopsis thaliana through proteomics will contribute to a deeper understanding of induced defense responses. As such, structurally characterized LPS chemotypes from Xanthomonas campestris pv. campestris (Xcc) wild-type 8004 (prototypical smooth-type LPS) and mutant 8530 (truncated core with no O–chain) strains were utilized to pre-treat A. thaliana plants. The associated proteomic response/changes within the PM were compared over a 24 h period using mass spectrometry-based methodologies following three variants of LPS-immobilized affinity chromatography. This resulted in the identification of proteins from several functional categories, but importantly, those involved in perception and defense. The distinct structural features between wild-type and mutant LPS are likely responsible for the differential changes to the proteome profiles, and many of the significant proteins were identified in response to the wild-type Xcc LPS where it is suggested that the core oligosaccharide and O-chain participate in recognition by receptor-like kinases (RLKs) in a multiprotein complex and, notably, varied from that of the mutant chemotype.

Original languageEnglish
Article number787
Pages (from-to)1-23
Number of pages23
JournalPathogens
Volume9
Issue number10
DOIs
Publication statusPublished - Oct 2020

Keywords

  • Affinity chromatography; innate immunity; lipopolysaccharide (LPS)
  • Pattern recognition receptors (PRRs)
  • Plasma membrane (PM)
  • Proteomics

ASJC Scopus subject areas

  • Immunology and Allergy
  • Molecular Biology
  • General Immunology and Microbiology
  • Microbiology (medical)
  • Infectious Diseases

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