TY - JOUR
T1 - Identification of hydroxylcinnamoyl tartaric acid esters in Bidens pilosa by UPLC-tandem mass spectrometry
AU - Khoza, B. S.
AU - Gbashi, S.
AU - Steenkamp, P. A.
AU - Njobeh, P. B.
AU - Madala, N. E.
N1 - Publisher Copyright:
© 2015 South African Association of Botanists.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Bidens pilosa is a medicinal plant used for the treatment of several physiological illnesses. In South Africa, as the case may be for other African countries, this plant is equally consumed as a vegetable. In the current study, pressurized hot water extraction (PHWE) technology was employed for the extraction of polyphenolic compounds from leaves of B. pilosa under two different temperature conditions (100 and 150. °C). Accordingly, extraction of these compounds was made possible at 150. °C and analysis of these extracts using UPLC-qTOF-MS/MS revealed the presence of several hydoxylcinnamoyl tartaric acids. Here, different isomers of coutaric-, caftaric-, fertaric-, chicoric acid and caftaric acid glycosides were detected. The contribution of mass spectrometry fragmentation towards the characterization of these molecules is also presented. To the best of our knowledge, this is the first report of these molecules in B. pilosa.
AB - Bidens pilosa is a medicinal plant used for the treatment of several physiological illnesses. In South Africa, as the case may be for other African countries, this plant is equally consumed as a vegetable. In the current study, pressurized hot water extraction (PHWE) technology was employed for the extraction of polyphenolic compounds from leaves of B. pilosa under two different temperature conditions (100 and 150. °C). Accordingly, extraction of these compounds was made possible at 150. °C and analysis of these extracts using UPLC-qTOF-MS/MS revealed the presence of several hydoxylcinnamoyl tartaric acids. Here, different isomers of coutaric-, caftaric-, fertaric-, chicoric acid and caftaric acid glycosides were detected. The contribution of mass spectrometry fragmentation towards the characterization of these molecules is also presented. To the best of our knowledge, this is the first report of these molecules in B. pilosa.
KW - Bidens pilosa
KW - Cinnamic acid derivatives
KW - Hydroxylcinnamoyl tartaric acids
KW - UPLC-qTOF-MS/MS
UR - http://www.scopus.com/inward/record.url?scp=84948440319&partnerID=8YFLogxK
U2 - 10.1016/j.sajb.2015.08.018
DO - 10.1016/j.sajb.2015.08.018
M3 - Article
AN - SCOPUS:84948440319
SN - 0254-6299
VL - 103
SP - 95
EP - 100
JO - South African Journal of Botany
JF - South African Journal of Botany
ER -
