Identification and quantification of gossypol in cotton by using packed micro-tips columns in combination with HPLC

R. Meyer, S. Vorster, I. A. Dubery

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Self-packed micro-tip columns containing a C18-bonded silica stationary phase, based on the same principles as solid-phase extraction methods, were used to obtain gossypol and related sesquiterpenoid aldehyde-enriched fractions. The enriched metabolite fractions were then analyzed by optimized high-performance liquid chromatography (HPLC) with a C18 column (4.6 mmx25 cm) eluted with the binary mobile phase acetonitrile-0.1% aqueous TFA solution (80:20). This method has proven to be highly reproducible. The precision and accuracy, as %RSD and %RME values, were determined to be less than 15% for the method. The minimum detection limit of gossypol was determined to be 10 ng (absolute gossypol). Absolute recovery was greater than 94% with a standard deviation of ± 3.68%. This is a simple, fast, and cost-effective method for isolation, identification, and quantification of gossypol and related secondary metabolites. Comparative analysis of gossypol content was performed on different parts of the cotton plant (seeds, stems and leaves) of two different cultivars of Gossypium hirsutum L. (Acala1517-70 and OR19). The results indicate that the OR19 cv naturally contains higher gossypol levels than the Acala cv. It was also found that treatment of leaves with a Verticillium dahliae-derived elicitor induced production of deoxyhemigossypol rather than gossypol.

Original languageEnglish
Pages (from-to)719-724
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Issue number4
Publication statusPublished - Oct 2004


  • Diode array
  • Extraction methods
  • Gossypium spp.
  • Gossypol
  • HPLC
  • Micro-tip
  • Solid-phase micro-extraction

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry


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