Hybrid Bionanocomposite Containing Magnesium Hydroxide Nanoparticles Embedded in a Carboxymethyl Cellulose Hydrogel plus Silk Fibroin as a Scaffold for Wound Dressing Applications

Reza Eivazzadeh-Keihan, Farzane Khalili, Nastaran Khosropour, Hooman Aghamirza Moghim Aliabadi, Fateme Radinekiyan, Sima Sukhtezari, Ali Maleki, Hamid Madanchi, Michael R. Hamblin, Mohammad Mahdavi, Seyed Mohammad Amin Haramshahi, Ahmed Esmail Shalan, Senentxu Lanceros-Méndez

Research output: Contribution to journalArticlepeer-review

79 Citations (Scopus)

Abstract

Based on the promising biomedical developments in wound healing strategies, herein, a new nanobiocomposite scaffold was designed and presented by incorporation of carboxymethyl cellulose hydrogels prepared using epichlorohydrin as a cross-linking agent (CMC hydrogel), a natural silk fibroin (SF) protein, and magnesium hydroxide nanoparticles (Mg(OH)2 NPs). Biological evaluation of the CMC hydrogel/SF/Mg(OH)2 nanobiocomposite scaffold was conducted via in vitro cell viability assays and in vivo assays, red blood cell hemolysis, and antibiofilm assays. Considering the cell viability percentage of Hu02 cells (84.5%) in the presence of the prepared nanobiocomposite after 7 days, it was indicated that this new nanoscaffold was biocompatible. The signs of excellent hemocompatibility and the high antibacterial activity were observed due to the low-point hemolytic effect (8.3%) and high-level potential in constraining the P. aeruginosa biofilm formation with a low OD value (0.13). Moreover, in vivo wound healing assay results indicated that the wound healing method was faster in mice treated with the prepared nanobiocomposite scaffold (82.29%) than the control group (75.63%) in 12 days. Apart from the structural characterization of the CMC hydrogel/SF/Mg(OH)2 nanobiocomposite through FTIR, EDX, FESEM, and TG analyses, compressive mechanical tests, contact angle, porosity, and swelling ratio studies indicated that the combination of the CMC hydrogel structure with SF protein and Mg(OH)2 NPs could significantly impact Young's modulus (from 11.34 to 10.14 MPa), tensile strength (from 299.35 to 250.78 MPa), elongation at break (12.52 to 12.84%), hydrophilicity, and water uptake capacity (92.5%).

Original languageEnglish
Pages (from-to)33840-33849
Number of pages10
JournalACS applied materials & interfaces
Volume13
Issue number29
DOIs
Publication statusPublished - 28 Jul 2021
Externally publishedYes

Keywords

  • hydrogel
  • magnesium hydroxide
  • nanobiocomposite
  • nanoparticles
  • scaffold
  • silk fibroin

ASJC Scopus subject areas

  • General Materials Science

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