TY - JOUR
T1 - HPLC-DAD fingerprinting analysis, antioxidant activities of Tithonia diversifolia (Hemsl.) A. Gray leaves and its inhibition of key enzymes linked to Alzheimer's disease
AU - Ojo, Oluwafemi Adeleke
AU - Ojo, Adebola Busola
AU - Ajiboye, Basiru Olaitan
AU - Olaiya, Oluranti
AU - Okesola, Mary Abiola
AU - Boligon, Aline Augusti
AU - de Campos, Marli Matiko Anraku
AU - Oyinloye, Babatunji Emmanuel
AU - Kappo, Abidemi Paul
N1 - Publisher Copyright:
© 2018 The Authors
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Tithonia diversifolia (Hemsl.) A. Gray leaves have long been used to manage neurodegenerative diseases without scientific basis. This study characterized the phenolic constituents, evaluated the antioxidant properties of phenolic extracts from T. diversifolia leaves used as traditional medicine in Africa and its inhibition of key enzymes linked to Alzheimer's disease. The extract was rich in phenolic acids (gallic acid, chlorogenic acid, caffeic acid and p-coumaric acid) and flavonoids (apigenin) and had 1,1-diphenyl-2-picryl-hydrazil radical scavenging abilities (IC50 = 41.05 μg. mL−1), 2,2-Azino-bis3-ethylbenthiazoline-6sulphonic acid radical scavenging ability (IC50 = 33.51 μg. mL−1), iron chelation (IC50 = 38.50 μg. mL−1), reducing power (Fe3+- Fe2+) (7.34 AAEmg/100 g), inhibited acetylcholinesterase (IC50 = 39.27 μg mL−1) and butyrylcholinesterase (IC50 = 35.01 μg mL−1) activities. These results reveal the leaf as a rich source of phenolic compounds with antioxidant and cholinesterase inhibitory activity.
AB - Tithonia diversifolia (Hemsl.) A. Gray leaves have long been used to manage neurodegenerative diseases without scientific basis. This study characterized the phenolic constituents, evaluated the antioxidant properties of phenolic extracts from T. diversifolia leaves used as traditional medicine in Africa and its inhibition of key enzymes linked to Alzheimer's disease. The extract was rich in phenolic acids (gallic acid, chlorogenic acid, caffeic acid and p-coumaric acid) and flavonoids (apigenin) and had 1,1-diphenyl-2-picryl-hydrazil radical scavenging abilities (IC50 = 41.05 μg. mL−1), 2,2-Azino-bis3-ethylbenthiazoline-6sulphonic acid radical scavenging ability (IC50 = 33.51 μg. mL−1), iron chelation (IC50 = 38.50 μg. mL−1), reducing power (Fe3+- Fe2+) (7.34 AAEmg/100 g), inhibited acetylcholinesterase (IC50 = 39.27 μg mL−1) and butyrylcholinesterase (IC50 = 35.01 μg mL−1) activities. These results reveal the leaf as a rich source of phenolic compounds with antioxidant and cholinesterase inhibitory activity.
KW - Alzheimer's disease
KW - Antioxidant activity
KW - Cholinergic enzymes
KW - Tithonia diversifolia leaves
UR - http://www.scopus.com/inward/record.url?scp=85047152285&partnerID=8YFLogxK
U2 - 10.1016/j.toxrep.2018.05.003
DO - 10.1016/j.toxrep.2018.05.003
M3 - Article
AN - SCOPUS:85047152285
SN - 2214-7500
VL - 5
SP - 585
EP - 592
JO - Toxicology Reports
JF - Toxicology Reports
ER -
