High-Performance Liquid Chromatographic Determination of D-arabino -Hexos-2-ulose (D-Glucosone) in Irradiated Sugar Solutions: Application of the Method to Irradiated Mango

A strongly basic anion-exchange HPLC column coupled with an amperometric detector was used for the direct detection of a mutagenic compound, D-arabino-hexos-2-ulose, in γ-irradiated sugar solutions. The method was applied to an irradiated model fruit system as well as an irradiated whole fruit. Although D-arabino-hexos-2-ulose could be detected in the model system, no indication of its presence was found in the real fruit. This study represents the first direct analysis for radiolysis products in a whole fruit.