High-affinity binding of a protein-lipopolysaccharide phytotoxin from Verticillium dahliae to cotton membranes

Riaan Meyer; Ian A. Dubery

doi:10.1016/0014-5793(93)80730-I

High-affinity binding of a protein-lipopolysaccharide phytotoxin from Verticillium dahliae to cotton membranes

Riaan Meyer, Ian A. Dubery

Biochemistry

Agricultural Research Council

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The presence of a specific binding site for a protein-lipopolysaccharide (PLP) phytotoxin isolated from culture filtrates of Verticillium dahliae has been demonstrated in plasma membranes from cotton seedlings. The ¹²⁵I-labelled PLP was used as a ligand in binding assays with plasma membrane enriched fractions. Root tissue exhibited the highest amount of binding activity compared to hypocotyl and cotyledon tissue. Binding of the [¹²⁵I]PLP was saturable, reversible, and with an affinity (K_d = 1.42 × 10^-8 M) comparable with the concentration required for biological activity. A single class of binding site was found, and the maximal number of binding sites were estimated as 5.4 × 10^-15 mol μg protein.

Original language	English
Pages (from-to)	203-206
Number of pages	4
Journal	FEBS Letters
Volume	335
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(93)80730-I
Publication status	Published - 6 Dec 1993

Keywords

Binding site
Cotton
Gossypium hirsutum
Phytotoxin
Protein-lipopolysaccharide
Verticillium dahliae

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(93)80730-I

Cite this

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title = "High-affinity binding of a protein-lipopolysaccharide phytotoxin from Verticillium dahliae to cotton membranes",

abstract = "The presence of a specific binding site for a protein-lipopolysaccharide (PLP) phytotoxin isolated from culture filtrates of Verticillium dahliae has been demonstrated in plasma membranes from cotton seedlings. The 125I-labelled PLP was used as a ligand in binding assays with plasma membrane enriched fractions. Root tissue exhibited the highest amount of binding activity compared to hypocotyl and cotyledon tissue. Binding of the [125I]PLP was saturable, reversible, and with an affinity (Kd = 1.42 × 10-8 M) comparable with the concentration required for biological activity. A single class of binding site was found, and the maximal number of binding sites were estimated as 5.4 × 10-15 mol μg protein.",

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T1 - High-affinity binding of a protein-lipopolysaccharide phytotoxin from Verticillium dahliae to cotton membranes

AU - Meyer, Riaan

AU - Dubery, Ian A.

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N2 - The presence of a specific binding site for a protein-lipopolysaccharide (PLP) phytotoxin isolated from culture filtrates of Verticillium dahliae has been demonstrated in plasma membranes from cotton seedlings. The 125I-labelled PLP was used as a ligand in binding assays with plasma membrane enriched fractions. Root tissue exhibited the highest amount of binding activity compared to hypocotyl and cotyledon tissue. Binding of the [125I]PLP was saturable, reversible, and with an affinity (Kd = 1.42 × 10-8 M) comparable with the concentration required for biological activity. A single class of binding site was found, and the maximal number of binding sites were estimated as 5.4 × 10-15 mol μg protein.

AB - The presence of a specific binding site for a protein-lipopolysaccharide (PLP) phytotoxin isolated from culture filtrates of Verticillium dahliae has been demonstrated in plasma membranes from cotton seedlings. The 125I-labelled PLP was used as a ligand in binding assays with plasma membrane enriched fractions. Root tissue exhibited the highest amount of binding activity compared to hypocotyl and cotyledon tissue. Binding of the [125I]PLP was saturable, reversible, and with an affinity (Kd = 1.42 × 10-8 M) comparable with the concentration required for biological activity. A single class of binding site was found, and the maximal number of binding sites were estimated as 5.4 × 10-15 mol μg protein.

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KW - Gossypium hirsutum

KW - Phytotoxin

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High-affinity binding of a protein-lipopolysaccharide phytotoxin from Verticillium dahliae to cotton membranes

Abstract

Keywords

ASJC Scopus subject areas

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