TY - GEN
T1 - He-Ne laser irradiation stimulates proliferation and migration of diabetic wounded fibroblast cells
AU - Houreld, Nicolette
AU - Abrahamse, Heidi
PY - 2008
Y1 - 2008
N2 - Diabetes mellitus is characterized by hyperglycemia which results in damage to the body, in particular to blood vessels and nerves. Atrophy of fat and muscle tissue shared with sensory loss can result in pressure sores, combined with delayed wound healing which can lead to lower-limb amputation. A diabetic wound model was achieved by growing WS1 fibroblast cells in 22.6 mmol/l glucose and a wound was simulated by scraping the cell sheet. Diabetic wounded (DW) cells were irradiated on day 1, day 1 and 2 or day 1 and 4 with a He-Ne laser (632.8 nm) with 5 or 16 J/cm2. Unirradiated DW cells were used as controls. The effect due to irradiation was determined by looking at cellular migration, viability (Trypan blue) and proliferation (IL-6 and bFGF expression and neutral red retention). DW cells irradiated with 5 J/cm2 on day 1 as well as day 1 and 4 showed an increase in cellular migration with complete wound closure by day 4. There was no negative effect on cellular viability and cells were able to normalize their cellular function, with an increase in IL-6, bFGF expression and neutral red retention. DW cells irradiated with 16 J/cm 2 showed an inhibition in cellular migration and a decrease in cellular viability and proliferation. The cumulative effect of irradiation on 2 consecutive days with both 5 and 16 J/cm2 could be seen by the decrease in viability. Morphologically these cells showed an inhibition in migration and showed signs of stress. There was a decrease in proliferation in irradiated and unirradiated DW cells over time. It is evident from this study that there is a cumulative effect, and that enough time between irradiations should be given when irradiating in vitro. A fluence of 5 J/cm2 encouraged wound healing, while 16 J/cm2 was inhibitive. The expression of IL-6 and neutral red retention was dependent on the stage of wound healing, as there was a decrease over time, thus it is important when choosing what parameters to evaluate and when to measure a response as there may be little or no observable effect. Phototherapy using the correct laser parameters appear to be beneficial to diabetic wound healing. The next phase of this study will be to link the in vitro findings with a 3D skin model which closely reproduces an in vivo situation.
AB - Diabetes mellitus is characterized by hyperglycemia which results in damage to the body, in particular to blood vessels and nerves. Atrophy of fat and muscle tissue shared with sensory loss can result in pressure sores, combined with delayed wound healing which can lead to lower-limb amputation. A diabetic wound model was achieved by growing WS1 fibroblast cells in 22.6 mmol/l glucose and a wound was simulated by scraping the cell sheet. Diabetic wounded (DW) cells were irradiated on day 1, day 1 and 2 or day 1 and 4 with a He-Ne laser (632.8 nm) with 5 or 16 J/cm2. Unirradiated DW cells were used as controls. The effect due to irradiation was determined by looking at cellular migration, viability (Trypan blue) and proliferation (IL-6 and bFGF expression and neutral red retention). DW cells irradiated with 5 J/cm2 on day 1 as well as day 1 and 4 showed an increase in cellular migration with complete wound closure by day 4. There was no negative effect on cellular viability and cells were able to normalize their cellular function, with an increase in IL-6, bFGF expression and neutral red retention. DW cells irradiated with 16 J/cm 2 showed an inhibition in cellular migration and a decrease in cellular viability and proliferation. The cumulative effect of irradiation on 2 consecutive days with both 5 and 16 J/cm2 could be seen by the decrease in viability. Morphologically these cells showed an inhibition in migration and showed signs of stress. There was a decrease in proliferation in irradiated and unirradiated DW cells over time. It is evident from this study that there is a cumulative effect, and that enough time between irradiations should be given when irradiating in vitro. A fluence of 5 J/cm2 encouraged wound healing, while 16 J/cm2 was inhibitive. The expression of IL-6 and neutral red retention was dependent on the stage of wound healing, as there was a decrease over time, thus it is important when choosing what parameters to evaluate and when to measure a response as there may be little or no observable effect. Phototherapy using the correct laser parameters appear to be beneficial to diabetic wound healing. The next phase of this study will be to link the in vitro findings with a 3D skin model which closely reproduces an in vivo situation.
KW - Diabetic wound healing
KW - Laser induced stimulation
KW - Laser light dose dependence on biological response
UR - http://www.scopus.com/inward/record.url?scp=84884993429&partnerID=8YFLogxK
U2 - 10.1007/978-0-387-71809-5_21
DO - 10.1007/978-0-387-71809-5_21
M3 - Conference contribution
AN - SCOPUS:84884993429
SN - 9780387718088
T3 - Lecture Notes in Electrical Engineering
SP - 221
EP - 232
BT - Proceedings of Light-Activated Tissue Regeneration and Therapy Conference
T2 - 2nd Conference on "Light-Activated Tissue Regeneration and Therapy" an Engineering Conference International, ECI 2007
Y2 - 24 June 2007 through 29 June 2007
ER -
