Green Synthesis of Silver Nanoparticles Using Nymphae odorata Extract Incorporated Films and Antimicrobial Activity

Silver nanoparticles (AgNPs) synthesis was formulated by the green method using Nymphae odorata plant extract as reducing and capping agent. Plants offer a good platform for synthesizing nanoparticles (NPs) which can act as a non-toxic, natural capping/reducing agent and can convert Ag⁺ to Ag⁰. The 5 min reduction time (the extract contains a lot of reduction agents, which leads to quick synthesis and such can be a great advantage) is a best experimental condition for the effective biological synthesis of AgNPs. Sodium alginate films were doped by these NPs. The antimicrobial study of AgNPs and doped films were examined by Staphylococcus aureus and Escherichia coli. As a result, at very low concentration of about 25 µl of AgNPs was found to inhibit the entire bacterial strains studied and films also showed a similar result. The results confirmed the effectiveness of prepared AgNPs and films as an antibacterial agent. Hence, it can be used for nano-biotechnology, biomedical and industrial applications.