Grape seed extract-soluplus dispersion and its antioxidant activity

R. Rajakumari, Tatiana Volova, Oluwatobi Samuel Oluwafemi, S. Rajesh Kumar, Sabu Thomas, Nandakumar Kalarikkal

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)


Objective: The main objective of this work was to formulate a nanodispersion containing grape seed extract and analyzed its release profile, antioxidant potential of the prepared formulations. Methods: The grape seed extract (GSE) containing proanthocyanidins (PC’s) has been dispersed in polymer matrix soluplus (SOLU) by the freeze-drying method. The morphological analysis was carried out using atomic force microscopy (AFM), scanning electron microscopy (SEM) and Transmission electron microscopy (TEM). The in-vitro release of the nanodispersion formulations was evaluated by simulated intestinal fluid (SIF). The antioxidant activity of GSE and the formulation were evaluated by employing various in-vitro assays such as 2, 2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2, 2-diphenyl-1- picrylhydrazyl (DPPH), Ferric reducing antioxidant power (FRAP) and peroxidation inhibiting activity. Results: The formulation FIII (1:5) resulted in a stable formulation with a higher loading efficiency of 95.36%, a particle size of 69.90 nm, a polydispersity index of 0.154 and a zeta potential value of −82.10 mV. The antioxidant efficiency of GSE-SOLU evaluated by DPPH was found to be 96.7%. The ABTS and FRAP model exhibited a dose-dependent scavenging activity. Linoleic model of FIII formulation and GSE exhibited a 66.14 and 86.58% inhibition respectively at 200 µg/l. Conclusions: The main reason for excellent scavenging activity of the formulations can be attributed to the presence of monomeric, dimeric, oligomeric procyanidins and the phenolic group. The present work denotes that GSE constitutes a good source of PC’s and will be useful in the prevention and treatment of free radical related diseases.

Original languageEnglish
Pages (from-to)1219-1229
Number of pages11
JournalDrug Development and Industrial Pharmacy
Publication statusPublished - 2020


  • Grape seed extract
  • antioxidant
  • dispersion
  • freeze-drying
  • proanthocyanidins
  • soluplus

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry


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