Global DNA methylation status of colorectal cancer cells exposed to photodynamic therapy

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Introduction: DNA methylation and histone modifications are epigenetic mechanisms that allow heritable silencing of genes without introducing mutations or alterations to the coding sequences of the genes. Being an important regulator of gene transcription, DNA methylation and its role in carcinogenesis has received considerable attention recently. Although hypermethylation represses transcription of promoter regions of tumour suppressor genes has been extensively studied, global hypomethylation has also been identified as an oncogenic inducer. Photodynamic therapy (PDT) is the use of low intensity laser irradiation (LILI) in conjunction with a photosensitiser (PS), in this instance Zinc (II) Phthalocyanine (ZnPc), to treat cancer cells. A single wavelength is used to activate the PS, which in turn causes changes in cellular functions. DNA methhylation is an epigenetic regulator. The methylation status of a gene determines if it is expressed or silenced. Aim: This study aimed to determine if DNA methylation has an effect on PDT. Method: Cancer cells, demethylated and normal, were exposed to PDT with different incubation times. Cell viability, proliferation and morphology were measured. Results: The results indicate that when cells are exposed to PDT, a statistical significant decrease (P< 0.001) in viability is achieved with an incubation time of 24 hours. The viability of cells treated with a high concentration of the demethylating agent showed a statistical significant decrease (P<0.01) in viability when incubated for 24 hours. Conclusion: DNA methylation does have an effect on the efficiency of PDT depending on the incubation time of the PS.

Original languageEnglish
Title of host publicationProceedings of the 9th World Association for Laser Therapy Congress, WALT 2012
PublisherMEDIMOND s.r.l.
Pages1-4
Number of pages4
ISBN (Print)9788875876777
Publication statusPublished - 2012
Event9th World Association for Laser Therapy Congress, WALT 2012 - Surfers Paradise, QT Gold Coast, Australia
Duration: 28 Sept 201230 Sept 2012

Publication series

NameProceedings of the 9th World Association for Laser Therapy Congress, WALT 2012

Conference

Conference9th World Association for Laser Therapy Congress, WALT 2012
Country/TerritoryAustralia
CitySurfers Paradise, QT Gold Coast
Period28/09/1230/09/12

Keywords

  • DNA methylation
  • Low intensity laser irradiation
  • Photodynamic therapy

ASJC Scopus subject areas

  • Biomedical Engineering
  • Dermatology
  • Surgery

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