TY - JOUR
T1 - Functionalized magnetic nanoparticles for the separation and purification of proteins and peptides
AU - Eivazzadeh-Keihan, Reza
AU - Bahreinizad, Hossein
AU - Amiri, Zeinab
AU - Aliabadi, Hooman Aghamirza Moghim
AU - Salimi-Bani, Milad
AU - Nakisa, Athar
AU - Davoodi, Farahnaz
AU - Tahmasebi, Behnam
AU - Ahmadpour, Farnoush
AU - Radinekiyan, Fateme
AU - Maleki, Ali
AU - Hamblin, Michael R.
AU - Mahdavi, Mohammad
AU - Madanchi, Hamid
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/8
Y1 - 2021/8
N2 - Nanoparticles have been widely employed for different biomedical applications due to their remarkable chemical and physical properties. The surface of nanomaterials can be modified by functionalization to create a strong recognition and binding sites between biological macromolecules and the nanostructures. Scientists have used this fact in the purification and extraction of bio-macromolecules like proteins, especially using magnetic nanoparticles that can be manipulated by an external magnetic field. The functionalization agents have included synthetic and natural polymers, antibodies, biomolecules or zwitterionic materials, as well as different nanomaterials such as carbon-based nanomaterials and metal oxide nanoparticles. The binding with the target is directly influenced by the size, surface energy and charge of the particles. Hydrophobicity and hydrophilicity of the nanoparticles is another important consideration that affects the selective purification of proteins by decorated magnetic nanoparticles.
AB - Nanoparticles have been widely employed for different biomedical applications due to their remarkable chemical and physical properties. The surface of nanomaterials can be modified by functionalization to create a strong recognition and binding sites between biological macromolecules and the nanostructures. Scientists have used this fact in the purification and extraction of bio-macromolecules like proteins, especially using magnetic nanoparticles that can be manipulated by an external magnetic field. The functionalization agents have included synthetic and natural polymers, antibodies, biomolecules or zwitterionic materials, as well as different nanomaterials such as carbon-based nanomaterials and metal oxide nanoparticles. The binding with the target is directly influenced by the size, surface energy and charge of the particles. Hydrophobicity and hydrophilicity of the nanoparticles is another important consideration that affects the selective purification of proteins by decorated magnetic nanoparticles.
KW - Antibodies
KW - Biological macromolecules
KW - Carbon nanomaterials
KW - Functionalized polymers
KW - Magnetic nanoparticles
KW - Metal ion affinity
KW - Protein corona
KW - Protein purification
KW - Surface modification
UR - http://www.scopus.com/inward/record.url?scp=85105581453&partnerID=8YFLogxK
U2 - 10.1016/j.trac.2021.116291
DO - 10.1016/j.trac.2021.116291
M3 - Review article
AN - SCOPUS:85105581453
SN - 0165-9936
VL - 141
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
M1 - 116291
ER -