First report of association of begomovirus in yellow mosaic disease of bur cucumber in India

Shivani Khanna; Sonali Rana; Jitender Singh; Manisha Goyal; Pankaj Kumar; Neetu Singh; R. P. Pant; V. K. Baranwal

doi:10.1007/s42360-018-00107-4

First report of association of begomovirus in yellow mosaic disease of bur cucumber in India

Shivani Khanna, Sonali Rana, Jitender Singh, Manisha Goyal, Pankaj Kumar, Neetu Singh, R. P. Pant, V. K. Baranwal

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

In November- December, 2016–2017 Bur cucumber (Sicyos angulatus) plants samples showing severe symptom of yellowing, mosaic and leaf curling were collected from Horticulture research centre (HRC), Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut. Polymerase chain reaction using coat protein gene specific primer (ToLCVAV1F and ToLCVAV1R) gave an amplification of ~ 750 bp, similar to leaf curl virus reported on different crops and weeds. The gene was sequenced and submitted to GenBank with MF471344. BLAST analysis showed the association of Begomovirus with 99% nucleotide sequence identity with Tomato leaf curl Palampur virus (ToLCPalV) strain India: UP: Matera: Tomato [KF157552]. Phylogenetic analysis also supported the relationship of the obtained sequence with other begomoviruses as they clustered in a separate monophyletic cluster with ToLCPalV-Karnataka, India (KY564204, KY564205), India; ToLCPalV-Varanasi, India (FJ931537, GQ225738); ToLCPalV-Matera (KF157552), India and ToLCPalV-Allipur, India (HQ848383). Sequence Identity matrix with other begomovirus sequences was found to be in range between (78 and 99%). To our knowledge, this is the first confirmed evidence of a begomovirus infecting weed bur cucumber (Sicyos angulatus) in India.

Original language	English
Pages (from-to)	181-184
Number of pages	4
Journal	Indian Phytopathology
Volume	72
Issue number	1
DOIs	https://doi.org/10.1007/s42360-018-00107-4
Publication status	Published - 13 Mar 2019
Externally published	Yes

Keywords

Coat protein
Phylogenetic analysis
Tomato leaf curl New Delhi virus
Tomato leaf curl Palampur virus

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s42360-018-00107-4

Cite this

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title = "First report of association of begomovirus in yellow mosaic disease of bur cucumber in India",

abstract = "In November- December, 2016–2017 Bur cucumber (Sicyos angulatus) plants samples showing severe symptom of yellowing, mosaic and leaf curling were collected from Horticulture research centre (HRC), Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut. Polymerase chain reaction using coat protein gene specific primer (ToLCVAV1F and ToLCVAV1R) gave an amplification of ~ 750 bp, similar to leaf curl virus reported on different crops and weeds. The gene was sequenced and submitted to GenBank with MF471344. BLAST analysis showed the association of Begomovirus with 99% nucleotide sequence identity with Tomato leaf curl Palampur virus (ToLCPalV) strain India: UP: Matera: Tomato [KF157552]. Phylogenetic analysis also supported the relationship of the obtained sequence with other begomoviruses as they clustered in a separate monophyletic cluster with ToLCPalV-Karnataka, India (KY564204, KY564205), India; ToLCPalV-Varanasi, India (FJ931537, GQ225738); ToLCPalV-Matera (KF157552), India and ToLCPalV-Allipur, India (HQ848383). Sequence Identity matrix with other begomovirus sequences was found to be in range between (78 and 99%). To our knowledge, this is the first confirmed evidence of a begomovirus infecting weed bur cucumber (Sicyos angulatus) in India.",

keywords = "Coat protein, Phylogenetic analysis, Tomato leaf curl New Delhi virus, Tomato leaf curl Palampur virus",

author = "Shivani Khanna and Sonali Rana and Jitender Singh and Manisha Goyal and Pankaj Kumar and Neetu Singh and Pant, {R. P.} and Baranwal, {V. K.}",

note = "Publisher Copyright: {\textcopyright} 2019, Indian Phytopathological Society.",

year = "2019",

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doi = "10.1007/s42360-018-00107-4",

language = "English",

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T1 - First report of association of begomovirus in yellow mosaic disease of bur cucumber in India

AU - Khanna, Shivani

AU - Rana, Sonali

AU - Singh, Jitender

AU - Goyal, Manisha

AU - Kumar, Pankaj

AU - Singh, Neetu

AU - Pant, R. P.

AU - Baranwal, V. K.

PY - 2019/3/13

Y1 - 2019/3/13

N2 - In November- December, 2016–2017 Bur cucumber (Sicyos angulatus) plants samples showing severe symptom of yellowing, mosaic and leaf curling were collected from Horticulture research centre (HRC), Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut. Polymerase chain reaction using coat protein gene specific primer (ToLCVAV1F and ToLCVAV1R) gave an amplification of ~ 750 bp, similar to leaf curl virus reported on different crops and weeds. The gene was sequenced and submitted to GenBank with MF471344. BLAST analysis showed the association of Begomovirus with 99% nucleotide sequence identity with Tomato leaf curl Palampur virus (ToLCPalV) strain India: UP: Matera: Tomato [KF157552]. Phylogenetic analysis also supported the relationship of the obtained sequence with other begomoviruses as they clustered in a separate monophyletic cluster with ToLCPalV-Karnataka, India (KY564204, KY564205), India; ToLCPalV-Varanasi, India (FJ931537, GQ225738); ToLCPalV-Matera (KF157552), India and ToLCPalV-Allipur, India (HQ848383). Sequence Identity matrix with other begomovirus sequences was found to be in range between (78 and 99%). To our knowledge, this is the first confirmed evidence of a begomovirus infecting weed bur cucumber (Sicyos angulatus) in India.

AB - In November- December, 2016–2017 Bur cucumber (Sicyos angulatus) plants samples showing severe symptom of yellowing, mosaic and leaf curling were collected from Horticulture research centre (HRC), Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut. Polymerase chain reaction using coat protein gene specific primer (ToLCVAV1F and ToLCVAV1R) gave an amplification of ~ 750 bp, similar to leaf curl virus reported on different crops and weeds. The gene was sequenced and submitted to GenBank with MF471344. BLAST analysis showed the association of Begomovirus with 99% nucleotide sequence identity with Tomato leaf curl Palampur virus (ToLCPalV) strain India: UP: Matera: Tomato [KF157552]. Phylogenetic analysis also supported the relationship of the obtained sequence with other begomoviruses as they clustered in a separate monophyletic cluster with ToLCPalV-Karnataka, India (KY564204, KY564205), India; ToLCPalV-Varanasi, India (FJ931537, GQ225738); ToLCPalV-Matera (KF157552), India and ToLCPalV-Allipur, India (HQ848383). Sequence Identity matrix with other begomovirus sequences was found to be in range between (78 and 99%). To our knowledge, this is the first confirmed evidence of a begomovirus infecting weed bur cucumber (Sicyos angulatus) in India.

KW - Coat protein

KW - Phylogenetic analysis

KW - Tomato leaf curl New Delhi virus

KW - Tomato leaf curl Palampur virus

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JF - Indian Phytopathology

IS - 1

ER -

First report of association of begomovirus in yellow mosaic disease of bur cucumber in India

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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