Expression of the severe acute respiratory syndrome coronavirus 3a protein and the assembly of coronavirus-like particles in the Baculovirus expression system

Sehaam Khan; Mah Lee Ng; Yee Joo Tan

doi:10.1385/1-59745-393-5:35

Expression of the severe acute respiratory syndrome coronavirus 3a protein and the assembly of coronavirus-like particles in the Baculovirus expression system

Sehaam Khan
, Mah Lee Ng
, Yee Joo Tan

Water and Health Research Centre

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Citations (Scopus)

Abstract

The Bac-to-Bac Baculovirus expression system was used to generate a recombinant baculovirus capable of expressing the severe acute respiratory syndrome (SARS)-coronavirus (CoV) 3a protein. Using the same expression system, two structural proteins, membrane (M) and envelope (E), were co-expressed to form SARS-CoV virus-like particles (VLPs) within an insect cell. Expression of viral proteins was confirmed by Western blot analysis and the formation of VLPs was studied by transmission electron microscopy.

Original language	English
Title of host publication	Glycovirology Protocols
Editors	Richard J. Sugrue
Pages	35-50
Number of pages	16
DOIs	https://doi.org/10.1385/1-59745-393-5:35
Publication status	Published - 2 Feb 2007

Publication series

Name	Methods in Molecular Biology
Volume	379
ISSN (Print)	1064-3745

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

3a protein
Bac-to-Bac Baculovirus expression system
Envelope protein (E)
Membrane protein (M)
SARS-CoV
Severe acute respiratory syndrome coronavirus
Transmission electron microscopy
Virus-like particles (VLPs)
Western blot

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1385/1-59745-393-5:35

Cite this

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title = "Expression of the severe acute respiratory syndrome coronavirus 3a protein and the assembly of coronavirus-like particles in the Baculovirus expression system",

abstract = "The Bac-to-Bac Baculovirus expression system was used to generate a recombinant baculovirus capable of expressing the severe acute respiratory syndrome (SARS)-coronavirus (CoV) 3a protein. Using the same expression system, two structural proteins, membrane (M) and envelope (E), were co-expressed to form SARS-CoV virus-like particles (VLPs) within an insect cell. Expression of viral proteins was confirmed by Western blot analysis and the formation of VLPs was studied by transmission electron microscopy.",

keywords = "3a protein, Bac-to-Bac Baculovirus expression system, Envelope protein (E), Membrane protein (M), SARS-CoV, Severe acute respiratory syndrome coronavirus, Transmission electron microscopy, Virus-like particles (VLPs), Western blot",

author = "Sehaam Khan and Ng, \{Mah Lee\} and Tan, \{Yee Joo\}",

year = "2007",

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Expression of the severe acute respiratory syndrome coronavirus 3a protein and the assembly of coronavirus-like particles in the Baculovirus expression system. / Khan, Sehaam; Ng, Mah Lee; Tan, Yee Joo.
Glycovirology Protocols. ed. / Richard J. Sugrue. 2007. p. 35-50 (Methods in Molecular Biology; Vol. 379).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

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KW - Membrane protein (M)

KW - SARS-CoV

KW - Severe acute respiratory syndrome coronavirus

KW - Transmission electron microscopy

KW - Virus-like particles (VLPs)

KW - Western blot

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Expression of the severe acute respiratory syndrome coronavirus 3a protein and the assembly of coronavirus-like particles in the Baculovirus expression system

Abstract

Publication series

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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