@inbook{ccc7d17af51c408b8226c3aa888fc5e0,
title = "Expression of the severe acute respiratory syndrome coronavirus 3a protein and the assembly of coronavirus-like particles in the Baculovirus expression system",
abstract = "The Bac-to-Bac Baculovirus expression system was used to generate a recombinant baculovirus capable of expressing the severe acute respiratory syndrome (SARS)-coronavirus (CoV) 3a protein. Using the same expression system, two structural proteins, membrane (M) and envelope (E), were co-expressed to form SARS-CoV virus-like particles (VLPs) within an insect cell. Expression of viral proteins was confirmed by Western blot analysis and the formation of VLPs was studied by transmission electron microscopy.",
keywords = "3a protein, Bac-to-Bac Baculovirus expression system, Envelope protein (E), Membrane protein (M), SARS-CoV, Severe acute respiratory syndrome coronavirus, Transmission electron microscopy, Virus-like particles (VLPs), Western blot",
author = "Sehaam Khan and Ng, {Mah Lee} and Tan, {Yee Joo}",
year = "2007",
month = feb,
day = "2",
doi = "10.1385/1-59745-393-5:35",
language = "English",
isbn = "1597453935",
series = "Methods in Molecular Biology",
pages = "35--50",
editor = "Sugrue, {Richard J.}",
booktitle = "Glycovirology Protocols",
}
