TY - JOUR
T1 - Expression of selected long non-coding RNAs in gastric cancer cells treated with coumarin
T2 - Possible mechanisms for anti-cancer activity
AU - Shaemi, Fatemeh
AU - Nejati, Majid
AU - Sarrafnia, Haleh
AU - Mahabady, Mahmood Khaksary
AU - Tamtaji, Zeinab
AU - Taheri, Abdolkarim Talebi
AU - Hamblin, Michael R.
AU - Zolfaghari, Mohammad Reza
AU - Heydari, Azhdar
AU - Mirzaei, Hamed
N1 - Publisher Copyright:
© 2023 Elsevier GmbH
PY - 2023/12
Y1 - 2023/12
N2 - Long non-coding RNAs (lncRNAs) can be utilized as prognostic indicators of gastric cancer since they can affect several cancer-related processes. Coumarin is a natural product with some useful anti-cancer properties. Here, we measured the expression of selected lncRNAs (RuPAR, SNHG6, CASC11, and their targets, miR-340–5p, p21, E-cadherin, and CDK1) in AGS gastric cancer cells treated with coumarin. MTT test has been utilized for assessing the AGS cells’ cell viability after exposure to coumarin. The expression of the lncRNAs (RuPAR, SNHG6, and CASC11) and miR-340–5p was evaluated via qRT-PCR. Western blot analysis has been utilized to determine changes in p21, E-cadherin, and CDK1 expression. Coumarin decreased AGS viability in a dose-dependent manner. The coumarin treated cells had lower levels of the mRNAs known to be targets of lncRNAs SNHG6 and CASC11 compared to control. Additionally, the coumarin group had increased levels of lncRNA RuPAR expression when compared with the control group. Some lncRNA targets, including p21, E-cadherin, and CDK1, showed lower expression in the coumarin group compared to the control by Western blotting. Coumarin could be a promising pharmacological candidate to be included in gastric cancer treatment regimens because it modulates lncRNAs and their targets.
AB - Long non-coding RNAs (lncRNAs) can be utilized as prognostic indicators of gastric cancer since they can affect several cancer-related processes. Coumarin is a natural product with some useful anti-cancer properties. Here, we measured the expression of selected lncRNAs (RuPAR, SNHG6, CASC11, and their targets, miR-340–5p, p21, E-cadherin, and CDK1) in AGS gastric cancer cells treated with coumarin. MTT test has been utilized for assessing the AGS cells’ cell viability after exposure to coumarin. The expression of the lncRNAs (RuPAR, SNHG6, and CASC11) and miR-340–5p was evaluated via qRT-PCR. Western blot analysis has been utilized to determine changes in p21, E-cadherin, and CDK1 expression. Coumarin decreased AGS viability in a dose-dependent manner. The coumarin treated cells had lower levels of the mRNAs known to be targets of lncRNAs SNHG6 and CASC11 compared to control. Additionally, the coumarin group had increased levels of lncRNA RuPAR expression when compared with the control group. Some lncRNA targets, including p21, E-cadherin, and CDK1, showed lower expression in the coumarin group compared to the control by Western blotting. Coumarin could be a promising pharmacological candidate to be included in gastric cancer treatment regimens because it modulates lncRNAs and their targets.
KW - CASC11
KW - Coumarin
KW - Gastric cancer
KW - Long non-coding RNAs
KW - MiR-340–5p
KW - RuPAR
KW - SNHG6
UR - http://www.scopus.com/inward/record.url?scp=85177878134&partnerID=8YFLogxK
U2 - 10.1016/j.prp.2023.154914
DO - 10.1016/j.prp.2023.154914
M3 - Article
C2 - 37992506
AN - SCOPUS:85177878134
SN - 0344-0338
VL - 252
JO - Pathology Research and Practice
JF - Pathology Research and Practice
M1 - 154914
ER -
