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Exploring the Therapeutic Potential of Cannabis Sativa: Phytochemical Profiling, Antioxidant, and Antimicrobial Activities of Two Distinct Strains

  • Folajimi T. Avoseh
  • , Samkeliso Takaidza
  • , Ochuko L. Erukainure
  • , Opeyemi N. Avoseh
  • , Fanyana M. Mtunzi
  • , Mpho G. Nkhi
  • Vaal University of Technology
  • University of KwaZulu-Natal
  • Lagos State University

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Cannabis sativa L. is increasingly recognized for its therapeutic potential beyond its psychoactive properties and use in fiber production. Recent research highlights its efficacy in treating pain, anxiety, inflammation, and as an antioxidant. These benefits are attributed to a variety of bioactive compounds, including cannabinoids, terpenes, and flavonoids, which have sparked interest in their medicinal applications. This study investigated the phytochemical composition and biological activities of two C. sativa strains, with a particular focus on identifying functional groups using FTIR. Crude extracts from C. sativa buds were prepared using solvents of varying polarities. Phytochemical contents were quantified using standard techniques. Antioxidant activity was measured via TLC, DPPH, and FRAP assays, while antimicrobial activity was assessed using the agar well diffusion method. FTIR analysis was used to detect functional groups in the extracts. Phytochemical analysis showed TPC values ranging from 11.77 to 93.51 mg GAE/g and TFC values from 25.61 to 45.95 mg QE/g. The ethyl acetate extract (high THC) exhibited the highest TPC (93.5 mg GAE/g) and a DPPH IC50 of 0.0065 µg/L, indicating strong antioxidant activity. Cheese 2 extracts (ethanolic and n-hexane) showed higher TFC values (45.95 & 45.59 mg QE/g). TLC confirmed antioxidant activity. The DPPH assay confirmed strong radical scavenging activity, while FRAP displayed notable activities in neutralizing free radicals. Both strains displayed notable antimicrobial activity against E. coli, S. aureus, B. cereus, and S. typhi. FTIR analysis identified key functional groups related to cannabinoids, terpenes, and fatty acids. These findings highlight C. sativa as a promising source of bioactive compounds with antioxidant and antimicrobial properties. However, further research is needed to fully assess its antioxidant potential.

Original languageEnglish
Title of host publicationProceedings of the 2nd International Conference on Futuristic Materials for Sustainable Development Goals - FMSDG 2025
EditorsSekomeng Johannes Modise, Mbuso Mlambo, Agnes Pholosi, Mokae Bambo, Kgomotso N. G. Mtshatsheni, Teboho Clement Mokhena, Langelihle Dlamini
PublisherSpringer Science and Business Media Deutschland GmbH
Pages23-43
Number of pages21
ISBN (Print)9789819519903
DOIs
Publication statusPublished - 2026
Externally publishedYes
Event2nd International Conference on Futuristic Materials for Sustainable Development Goals, FMSDG 2025 - Vanderbijlpark, South Africa
Duration: 6 Apr 20259 Apr 2025

Publication series

NameSpringer Proceedings in Physics
Volume434 SPPHY
ISSN (Print)0930-8989
ISSN (Electronic)1867-4941

Conference

Conference2nd International Conference on Futuristic Materials for Sustainable Development Goals, FMSDG 2025
Country/TerritorySouth Africa
CityVanderbijlpark
Period6/04/259/04/25

Keywords

  • Antimicrobial
  • Antioxidant
  • Bioactive compounds
  • Cannabis sativa
  • Phytochemistry

ASJC Scopus subject areas

  • General Physics and Astronomy

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