Exfoliative liver cytology in oreochromis mossambicus as a bio-assessment tool for aquatic pollution

M. L. Mokae; J. H.J. van Vuren; G. M. Pieterse

doi:10.2989/16085914.2010.466641

Exfoliative liver cytology in oreochromis mossambicus as a bio-assessment tool for aquatic pollution

M. L. Mokae, J. H.J. van Vuren, G. M. Pieterse

Zoology

University of Johannesburg

Research output: Contribution to journal › Article › peer-review

Abstract

The use of exfoliative cytology as a bio-assessment tool was evaluated to (1) identify and describe different cell types of normal liver cytology of Oreochromis mossambicus, (2) identify and describe changes in the cell types after exposure to cadmium and zinc, and (3) determine if non-imaging-guided fine-needle aspiration (FNA) cytology can be performed without sacrificing the fish. All fish used in the aspiration experiment recovered and survived. Livers of O. mossambicus exposed to cadmium and zinc showed excess numbers of exfoliated red blood cells, loss of cellular cohesion, regenerative hyperplasia, pale and weak cytoplasm staining, pronounced vacuolation and degeneration. Cadmium and zinc exposure induced pathological cellular changes. Exfoliative cytology is thus a safe, cost-effective and reliable bio-assessment tool.

Original language	English
Pages (from-to)	37-46
Number of pages	10
Journal	African Journal of Aquatic Science
Volume	35
Issue number	1
DOIs	https://doi.org/10.2989/16085914.2010.466641
Publication status	Published - May 2010

Keywords

Cadmium
Hepatocytes
Non-imaging-guided FNA
Zinc

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Aquatic Science

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10.2989/16085914.2010.466641

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Exfoliative liver cytology in oreochromis mossambicus as a bio-assessment tool for aquatic pollution

Abstract

Keywords

ASJC Scopus subject areas

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