Evidence for a cryptic RNA polymerase activity in DNA-free minicells of Escherichia coli

Allen C. Rogerson

doi:10.1016/0006-291X(75)90561-6

Evidence for a cryptic RNA polymerase activity in DNA-free minicells of Escherichia coli

Allen C. Rogerson

University of Copenhagen

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

DNA-free minicells of Escherichia coli will not allow growth of phage T-7, nor is RNA synthesis stimulated by phage infection. Thus, these miniature cells seem not to contain in vivo RNA polymerase activity. However, DNA-dependent RNA polymerase activity can be unmasked in extracts with poly(dA-T) and Mn²⁺. This activity may represent a cytoplasmic pool of inactive RNA polymerase in normal cells.

Original language	English
Pages (from-to)	665-670
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	66
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(75)90561-6
Publication status	Published - 16 Sept 1975
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0006-291X(75)90561-6

Cite this

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title = "Evidence for a cryptic RNA polymerase activity in DNA-free minicells of Escherichia coli",

abstract = "DNA-free minicells of Escherichia coli will not allow growth of phage T-7, nor is RNA synthesis stimulated by phage infection. Thus, these miniature cells seem not to contain in vivo RNA polymerase activity. However, DNA-dependent RNA polymerase activity can be unmasked in extracts with poly(dA-T) and Mn2+. This activity may represent a cytoplasmic pool of inactive RNA polymerase in normal cells.",

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AU - Rogerson, Allen C.

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Y1 - 1975/9/16

N2 - DNA-free minicells of Escherichia coli will not allow growth of phage T-7, nor is RNA synthesis stimulated by phage infection. Thus, these miniature cells seem not to contain in vivo RNA polymerase activity. However, DNA-dependent RNA polymerase activity can be unmasked in extracts with poly(dA-T) and Mn2+. This activity may represent a cytoplasmic pool of inactive RNA polymerase in normal cells.

AB - DNA-free minicells of Escherichia coli will not allow growth of phage T-7, nor is RNA synthesis stimulated by phage infection. Thus, these miniature cells seem not to contain in vivo RNA polymerase activity. However, DNA-dependent RNA polymerase activity can be unmasked in extracts with poly(dA-T) and Mn2+. This activity may represent a cytoplasmic pool of inactive RNA polymerase in normal cells.

UR - https://www.scopus.com/pages/publications/0016793997

U2 - 10.1016/0006-291X(75)90561-6

DO - 10.1016/0006-291X(75)90561-6

M3 - Article

C2 - 1101890

AN - SCOPUS:0016793997

SN - 0006-291X

VL - 66

SP - 665

EP - 670

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Evidence for a cryptic RNA polymerase activity in DNA-free minicells of Escherichia coli

Abstract

ASJC Scopus subject areas

UN SDGs

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