Evidence for a cryptic RNA polymerase activity in DNA-free minicells of Escherichia coli

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7 Citations (Scopus)

Abstract

DNA-free minicells of Escherichia coli will not allow growth of phage T-7, nor is RNA synthesis stimulated by phage infection. Thus, these miniature cells seem not to contain in vivo RNA polymerase activity. However, DNA-dependent RNA polymerase activity can be unmasked in extracts with poly(dA-T) and Mn2+. This activity may represent a cytoplasmic pool of inactive RNA polymerase in normal cells.

Original languageEnglish
Pages (from-to)665-670
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume66
Issue number2
DOIs
Publication statusPublished - 16 Sept 1975
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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