Abstract
DNA-free minicells of Escherichia coli will not allow growth of phage T-7, nor is RNA synthesis stimulated by phage infection. Thus, these miniature cells seem not to contain in vivo RNA polymerase activity. However, DNA-dependent RNA polymerase activity can be unmasked in extracts with poly(dA-T) and Mn2+. This activity may represent a cytoplasmic pool of inactive RNA polymerase in normal cells.
Original language | English |
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Pages (from-to) | 665-670 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 66 |
Issue number | 2 |
DOIs | |
Publication status | Published - 16 Sept 1975 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology