Electrochemical immunosensor based on polythionine/gold nanoparticles for the determination of aflatoxin B1

Joseph H.O. Owino; Omotayo A. Arotiba; Nicolette Hendricks; Everlyne A. Songa; Nazeem Jahed; Tesfaye T. Waryo; Rachel F. Ngece; Priscilla G.L. Baker; Emmanuel I. Iwuoha

doi:10.3390/s8128262

Electrochemical immunosensor based on polythionine/gold nanoparticles for the determination of aflatoxin B₁

Joseph H.O. Owino, Omotayo A. Arotiba, Nicolette Hendricks, Everlyne A. Songa, Nazeem Jahed, Tesfaye T. Waryo, Rachel F. Ngece, Priscilla G.L. Baker, Emmanuel I. Iwuoha

University of the Western Cape

Research output: Contribution to journal › Article › peer-review

104 Citations (Scopus)

Abstract

An aflatoxin B₁ (AFB₁) electrochemical immunosensor was developed by the immobilisation of aflatoxin B₁-bovine serum albumin (AFB₁-BSA) conjugate on a polythionine (PTH)/gold nanoparticles (AuNP)-modified glassy carbon electrode (GCE). The surface of the AFB₁-BSA conjugate was covered with horseradish peroxidase (HRP), in order to prevent non-specific binding of the immunosensors with ions in the test solution. The AFB₁ immunosensor exhibited a quasi-reversible electrochemistry as indicated by a cyclic voltammetric (CV) peak separation (ΔE_P) value of 62 mV. The experimental procedure for the detection of AFB₁ involved the setting up of a competition between free AFB₁ and the immobilised AFB₁-BSA conjugate for the binding sites of free anti-aflatoxin B₁ (anti-AFB₁) antibody. The immunosensor's differential pulse voltammetry (DPV) responses (peak currents) decreased as the concentration of free AFB₁ increased within a dynamic linear range (DLR) of 0.6 - 2.4 ng/mL AFB₁ and a limit of detection (LOD) of 0.07 ng/mL AFB₁. This immunosensing procedure eliminates the need for enzyme-labeled secondary antibodies normally used in conventional ELISA-based immunosensors.

Original language	English
Pages (from-to)	8262-8274
Number of pages	13
Journal	Sensors
Volume	8
Issue number	12
DOIs	https://doi.org/10.3390/s8128262
Publication status	Published - Dec 2008
Externally published	Yes

Keywords

Aflatoxin B
Gold nanoparticles
Horseradish peroxidise (HRP)
Immunosensor
Polythionine

ASJC Scopus subject areas

Analytical Chemistry
Information Systems
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering

Access to Document

10.3390/s8128262

Cite this

@article{db3829825d3d452ab400bcf9f6767114,

title = "Electrochemical immunosensor based on polythionine/gold nanoparticles for the determination of aflatoxin B1",

abstract = "An aflatoxin B1 (AFB1) electrochemical immunosensor was developed by the immobilisation of aflatoxin B1-bovine serum albumin (AFB1-BSA) conjugate on a polythionine (PTH)/gold nanoparticles (AuNP)-modified glassy carbon electrode (GCE). The surface of the AFB1-BSA conjugate was covered with horseradish peroxidase (HRP), in order to prevent non-specific binding of the immunosensors with ions in the test solution. The AFB1 immunosensor exhibited a quasi-reversible electrochemistry as indicated by a cyclic voltammetric (CV) peak separation (ΔEP) value of 62 mV. The experimental procedure for the detection of AFB1 involved the setting up of a competition between free AFB1 and the immobilised AFB1-BSA conjugate for the binding sites of free anti-aflatoxin B1 (anti-AFB1) antibody. The immunosensor's differential pulse voltammetry (DPV) responses (peak currents) decreased as the concentration of free AFB1 increased within a dynamic linear range (DLR) of 0.6 - 2.4 ng/mL AFB1 and a limit of detection (LOD) of 0.07 ng/mL AFB1. This immunosensing procedure eliminates the need for enzyme-labeled secondary antibodies normally used in conventional ELISA-based immunosensors.",

keywords = "Aflatoxin B, Gold nanoparticles, Horseradish peroxidise (HRP), Immunosensor, Polythionine",

author = "Owino, {Joseph H.O.} and Arotiba, {Omotayo A.} and Nicolette Hendricks and Songa, {Everlyne A.} and Nazeem Jahed and Waryo, {Tesfaye T.} and Ngece, {Rachel F.} and Baker, {Priscilla G.L.} and Iwuoha, {Emmanuel I.}",

year = "2008",

month = dec,

doi = "10.3390/s8128262",

language = "English",

volume = "8",

pages = "8262--8274",

journal = "Sensors",

issn = "1424-8220",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "12",

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TY - JOUR

T1 - Electrochemical immunosensor based on polythionine/gold nanoparticles for the determination of aflatoxin B1

AU - Owino, Joseph H.O.

AU - Arotiba, Omotayo A.

AU - Hendricks, Nicolette

AU - Songa, Everlyne A.

AU - Jahed, Nazeem

AU - Waryo, Tesfaye T.

AU - Ngece, Rachel F.

AU - Baker, Priscilla G.L.

AU - Iwuoha, Emmanuel I.

PY - 2008/12

Y1 - 2008/12

N2 - An aflatoxin B1 (AFB1) electrochemical immunosensor was developed by the immobilisation of aflatoxin B1-bovine serum albumin (AFB1-BSA) conjugate on a polythionine (PTH)/gold nanoparticles (AuNP)-modified glassy carbon electrode (GCE). The surface of the AFB1-BSA conjugate was covered with horseradish peroxidase (HRP), in order to prevent non-specific binding of the immunosensors with ions in the test solution. The AFB1 immunosensor exhibited a quasi-reversible electrochemistry as indicated by a cyclic voltammetric (CV) peak separation (ΔEP) value of 62 mV. The experimental procedure for the detection of AFB1 involved the setting up of a competition between free AFB1 and the immobilised AFB1-BSA conjugate for the binding sites of free anti-aflatoxin B1 (anti-AFB1) antibody. The immunosensor's differential pulse voltammetry (DPV) responses (peak currents) decreased as the concentration of free AFB1 increased within a dynamic linear range (DLR) of 0.6 - 2.4 ng/mL AFB1 and a limit of detection (LOD) of 0.07 ng/mL AFB1. This immunosensing procedure eliminates the need for enzyme-labeled secondary antibodies normally used in conventional ELISA-based immunosensors.

AB - An aflatoxin B1 (AFB1) electrochemical immunosensor was developed by the immobilisation of aflatoxin B1-bovine serum albumin (AFB1-BSA) conjugate on a polythionine (PTH)/gold nanoparticles (AuNP)-modified glassy carbon electrode (GCE). The surface of the AFB1-BSA conjugate was covered with horseradish peroxidase (HRP), in order to prevent non-specific binding of the immunosensors with ions in the test solution. The AFB1 immunosensor exhibited a quasi-reversible electrochemistry as indicated by a cyclic voltammetric (CV) peak separation (ΔEP) value of 62 mV. The experimental procedure for the detection of AFB1 involved the setting up of a competition between free AFB1 and the immobilised AFB1-BSA conjugate for the binding sites of free anti-aflatoxin B1 (anti-AFB1) antibody. The immunosensor's differential pulse voltammetry (DPV) responses (peak currents) decreased as the concentration of free AFB1 increased within a dynamic linear range (DLR) of 0.6 - 2.4 ng/mL AFB1 and a limit of detection (LOD) of 0.07 ng/mL AFB1. This immunosensing procedure eliminates the need for enzyme-labeled secondary antibodies normally used in conventional ELISA-based immunosensors.

KW - Aflatoxin B

KW - Gold nanoparticles

KW - Horseradish peroxidise (HRP)

KW - Immunosensor

KW - Polythionine

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