Electrochemical immunosensor based on polythionine/gold nanoparticles for the determination of aflatoxin B1

Joseph H.O. Owino, Omotayo A. Arotiba, Nicolette Hendricks, Everlyne A. Songa, Nazeem Jahed, Tesfaye T. Waryo, Rachel F. Ngece, Priscilla G.L. Baker, Emmanuel I. Iwuoha

Research output: Contribution to journalArticlepeer-review

103 Citations (Scopus)

Abstract

An aflatoxin B1 (AFB1) electrochemical immunosensor was developed by the immobilisation of aflatoxin B1-bovine serum albumin (AFB1-BSA) conjugate on a polythionine (PTH)/gold nanoparticles (AuNP)-modified glassy carbon electrode (GCE). The surface of the AFB1-BSA conjugate was covered with horseradish peroxidase (HRP), in order to prevent non-specific binding of the immunosensors with ions in the test solution. The AFB1 immunosensor exhibited a quasi-reversible electrochemistry as indicated by a cyclic voltammetric (CV) peak separation (ΔEP) value of 62 mV. The experimental procedure for the detection of AFB1 involved the setting up of a competition between free AFB1 and the immobilised AFB1-BSA conjugate for the binding sites of free anti-aflatoxin B1 (anti-AFB1) antibody. The immunosensor's differential pulse voltammetry (DPV) responses (peak currents) decreased as the concentration of free AFB1 increased within a dynamic linear range (DLR) of 0.6 - 2.4 ng/mL AFB1 and a limit of detection (LOD) of 0.07 ng/mL AFB1. This immunosensing procedure eliminates the need for enzyme-labeled secondary antibodies normally used in conventional ELISA-based immunosensors.

Original languageEnglish
Pages (from-to)8262-8274
Number of pages13
JournalSensors
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 2008
Externally publishedYes

Keywords

  • Aflatoxin B
  • Gold nanoparticles
  • Horseradish peroxidise (HRP)
  • Immunosensor
  • Polythionine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Information Systems
  • Atomic and Molecular Physics, and Optics
  • Biochemistry
  • Instrumentation
  • Electrical and Electronic Engineering

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