Electrochemical aptasensing of cadmium (II) on a carbon black-gold nano-platform

Colani T. Fakude, Omotayo A. Arotiba, Nonhlangabezo Mabuba

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

A study on the electrochemical aptasensing of Cd(II) on a screen printed carbon electrode (SPCE) modified with carbon black (CB) and gold nanoparticles (AuNPs) is reported. The nano-platform was prepared by drop casting CB and electrodepositing AuNPs by cycling potential from −400 mV to 1100 mV at 50 mVs−1 scan rate. The immobilized 36-base thiolated ssDNA aptamer on the SPCE-CB-AuNPs, upon interaction with Cd(II), underwent a conformational change that resulted in change in electrochemical current response. After the optimization of incubation time and aptamer concentration at 30 min and 2 μM, respectively, the aptasensor detected Cd(II) in the linear range of 1–50 ppb with a limit of detection of 0.14 ppb using square wave voltammetry (SWV). The aptasensor was applied in real water sample analysis and the results were validated with inductively coupled plasma-optimal emission spectroscope (ICP-OES). The aptasensor demonstrated good reproducibility, acceptable stability and excellent selectivity towards Cd(II), endorsing potential application in environmental monitoring of Cd(II).

Original languageEnglish
Article number113796
JournalJournal of Electroanalytical Chemistry
Volume858
DOIs
Publication statusPublished - 1 Feb 2020

Keywords

  • Biosensor, aptamer
  • Cadmium
  • Carbon black
  • Electrochemical aptasensor
  • Gold nanoparticles

ASJC Scopus subject areas

  • Analytical Chemistry
  • General Chemical Engineering
  • Electrochemistry

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