Effects of the phenotypic polarization state of human leukocytes on the optical absorbance spectrum

Kaline de Brito Sousa, Daniela de Fátima Teixeira da Silva, Maria Fernanda Setúbal Destro Rodrigues, Mónica Pereira Garcia, Carolina de Oliveira Rodini, Raquel Agnelli Mesquita-Ferrari, Michael R. Hamblin, Sandra Kalil Bussadori, Fabio Daumas Nunes, Kristianne Porta Santos Fernandes

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

This study evaluated the optical absorbance spectrum of human monocytes, neutrophils and lymphocytes polarized, or not, to the inflammatory or immunoregulatory phenotypes. Peripheral human blood leukocytes were isolated and polarized (10 ng/mL) with LPS or IL-4 + LPS for 2 hours. After polarization, cells were washed and incubated for an additional 24 hours (monocytes and lymphocytes) or 12 hours (neutrophils). Next, cells were collected to evaluate the optical absorbance spectrum. The three types of leukocytes exhibited absorbance in the region from 450 to 900 nm, with greater absorbance at wavelengths lower than 570 nm. Lymphocytes had a second region of greater absorbance between 770 and 900 nm. Inflammatory monocytes and lymphocytes showed increased absorbance of blue, green and yellow wavelengths (monocytes), as well as red and infrared wavelengths (monocytes and lymphocytes). Immunoregulatory polarization altered the absorbance of monocytes and lymphocytes very little. Neutrophils treated with LPS or LPS + IL-4 exhibited lower absorbance at wavelengths higher than 575 nm compared to untreated cells. The present findings showed that leukocytes exhibit greater absorbance in regions of the spectrum that have not been much used in photobiomodulation (PBM), and the polarization of these cells can affect their capacity to absorb light. Taken together, these results suggest new perspectives in the use of PBM in the clinical setting depending on the wavelengths and the stage of the inflammatory process.

Original languageEnglish
Article numbere202000487
JournalJournal of Biophotonics
Volume14
Issue number6
DOIs
Publication statusPublished - Jun 2021

Keywords

  • leukocytes
  • lymphocytes
  • monocytes
  • neutrophils
  • optical absorbance
  • photobiomodulation

ASJC Scopus subject areas

  • General Chemistry
  • General Materials Science
  • General Biochemistry,Genetics and Molecular Biology
  • General Engineering
  • General Physics and Astronomy

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