TY - JOUR
T1 - Effects of the phenotypic polarization state of human leukocytes on the optical absorbance spectrum
AU - de Brito Sousa, Kaline
AU - de Fátima Teixeira da Silva, Daniela
AU - Rodrigues, Maria Fernanda Setúbal Destro
AU - Garcia, Mónica Pereira
AU - de Oliveira Rodini, Carolina
AU - Mesquita-Ferrari, Raquel Agnelli
AU - Hamblin, Michael R.
AU - Bussadori, Sandra Kalil
AU - Nunes, Fabio Daumas
AU - Fernandes, Kristianne Porta Santos
N1 - Publisher Copyright:
© 2021 Wiley-VCH GmbH
PY - 2021/6
Y1 - 2021/6
N2 - This study evaluated the optical absorbance spectrum of human monocytes, neutrophils and lymphocytes polarized, or not, to the inflammatory or immunoregulatory phenotypes. Peripheral human blood leukocytes were isolated and polarized (10 ng/mL) with LPS or IL-4 + LPS for 2 hours. After polarization, cells were washed and incubated for an additional 24 hours (monocytes and lymphocytes) or 12 hours (neutrophils). Next, cells were collected to evaluate the optical absorbance spectrum. The three types of leukocytes exhibited absorbance in the region from 450 to 900 nm, with greater absorbance at wavelengths lower than 570 nm. Lymphocytes had a second region of greater absorbance between 770 and 900 nm. Inflammatory monocytes and lymphocytes showed increased absorbance of blue, green and yellow wavelengths (monocytes), as well as red and infrared wavelengths (monocytes and lymphocytes). Immunoregulatory polarization altered the absorbance of monocytes and lymphocytes very little. Neutrophils treated with LPS or LPS + IL-4 exhibited lower absorbance at wavelengths higher than 575 nm compared to untreated cells. The present findings showed that leukocytes exhibit greater absorbance in regions of the spectrum that have not been much used in photobiomodulation (PBM), and the polarization of these cells can affect their capacity to absorb light. Taken together, these results suggest new perspectives in the use of PBM in the clinical setting depending on the wavelengths and the stage of the inflammatory process.
AB - This study evaluated the optical absorbance spectrum of human monocytes, neutrophils and lymphocytes polarized, or not, to the inflammatory or immunoregulatory phenotypes. Peripheral human blood leukocytes were isolated and polarized (10 ng/mL) with LPS or IL-4 + LPS for 2 hours. After polarization, cells were washed and incubated for an additional 24 hours (monocytes and lymphocytes) or 12 hours (neutrophils). Next, cells were collected to evaluate the optical absorbance spectrum. The three types of leukocytes exhibited absorbance in the region from 450 to 900 nm, with greater absorbance at wavelengths lower than 570 nm. Lymphocytes had a second region of greater absorbance between 770 and 900 nm. Inflammatory monocytes and lymphocytes showed increased absorbance of blue, green and yellow wavelengths (monocytes), as well as red and infrared wavelengths (monocytes and lymphocytes). Immunoregulatory polarization altered the absorbance of monocytes and lymphocytes very little. Neutrophils treated with LPS or LPS + IL-4 exhibited lower absorbance at wavelengths higher than 575 nm compared to untreated cells. The present findings showed that leukocytes exhibit greater absorbance in regions of the spectrum that have not been much used in photobiomodulation (PBM), and the polarization of these cells can affect their capacity to absorb light. Taken together, these results suggest new perspectives in the use of PBM in the clinical setting depending on the wavelengths and the stage of the inflammatory process.
KW - leukocytes
KW - lymphocytes
KW - monocytes
KW - neutrophils
KW - optical absorbance
KW - photobiomodulation
UR - http://www.scopus.com/inward/record.url?scp=85102190794&partnerID=8YFLogxK
U2 - 10.1002/jbio.202000487
DO - 10.1002/jbio.202000487
M3 - Article
C2 - 33638279
AN - SCOPUS:85102190794
SN - 1864-063X
VL - 14
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 6
M1 - e202000487
ER -