Effect of wavelength and fluence on morphology, cellular and genetic integrity of diabetic wounded human skin fibroblasts

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

11 Citations (Scopus)

Abstract

An alternative treatment modality for diabetic wound healing includes low level laser therapy (LLLT). Biostimulation of such wounds may be of benefit to patients by reducing healing time. Structural, cellular and genetic events in diabetic wounded human skin fibroblasts (WS1) were evaluated after exposing cells in culture to a Helium-Neon (632.8nm), a Diode laser (830nm) and a Nd:YAG (Neodynium:Yttrium-Allumina-Gallium) laser (1064nm) at either 5J/cm2 or 16J/cm2. Cells were exposed twice a week and left 24 hours post-irradiation prior to measuring effects. Structural changes were evaluated by assessing colony formation, haptotaxis and chemotaxis. Cellular changes were evaluated using cell viability, (adenosine-triphosphate, ATP production), and proliferation, (alkaline phosphatase, ALP and basic fibroblast growth factor, bFGF expression), while the Comet assay evaluated DNA damage and cytotoxicity was determined assessing membrane permeability for lactate dehydrogenase (LDH). Caspase 3/7 activity was used as an estimate of apoptosis as a result of irradiation. The irradiated diabetic wounded cells showed structural, cellular as well as molecular resilience comparable to that of unwounded normal skin fibroblast cells. With regards to fluence, 5J/cm2 elicit positive cellular and structural responses while 16J/cm2 increases cellular and genetic damage and cellular morphology is altered. Different wavelengths of LLLT influences the beneficial outcomes of diabetic wounded cells and although all three wavelengths elicit cellular effects, the penetration depth of 830nm plays a significant role in the healing of diabetic wounded human fibroblast cells. Results from this study validate the contribution of LLLT to wound healing and elucidate the biochemical effects at a cellular level while highlighting the role of different dosages and wavelengths in LLLT.

Original languageEnglish
Title of host publicationMechanisms for Low-Light Therapy
DOIs
Publication statusPublished - 2006
EventMechanisms for Low-Light Therapy - San Jose, CA, United States
Duration: 22 Jan 200624 Jan 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6140
ISSN (Print)1605-7422

Conference

ConferenceMechanisms for Low-Light Therapy
Country/TerritoryUnited States
CitySan Jose, CA
Period22/01/0624/01/06

Keywords

  • DNA damage
  • Diabetic wound healing
  • Fluence
  • LLLT
  • Proliferation
  • Viability
  • Wavelength

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology, Nuclear Medicine and Imaging

Fingerprint

Dive into the research topics of 'Effect of wavelength and fluence on morphology, cellular and genetic integrity of diabetic wounded human skin fibroblasts'. Together they form a unique fingerprint.

Cite this