Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells

B. Mvula; T. J. Moore; H. Abrahamse

doi:10.1007/s10103-008-0636-1

Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells

B. Mvula, T. J. Moore, H. Abrahamse

Laser Research Centre

University of Johannesburg

Research output: Contribution to journal › Article › peer-review

98 Citations (Scopus)

Abstract

The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm² using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm² on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.

Original language	English
Pages (from-to)	33-39
Number of pages	7
Journal	Lasers in Medical Science
Volume	25
Issue number	1
DOIs	https://doi.org/10.1007/s10103-008-0636-1
Publication status	Published - Jan 2010

Keywords

Epidermal growth factor
Low-level laser
Proliferation
Stem cells
Viability

ASJC Scopus subject areas

Surgery
Dermatology

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10.1007/s10103-008-0636-1

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abstract = "The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm2 using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm2 on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.",

keywords = "Epidermal growth factor, Low-level laser, Proliferation, Stem cells, Viability",

author = "B. Mvula and Moore, \{T. J.\} and H. Abrahamse",

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doi = "10.1007/s10103-008-0636-1",

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AU - Mvula, B.

AU - Moore, T. J.

AU - Abrahamse, H.

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N2 - The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm2 using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm2 on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.

AB - The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm2 using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm2 on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.

KW - Epidermal growth factor

KW - Low-level laser

KW - Proliferation

KW - Stem cells

KW - Viability

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Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells

Abstract

Keywords

ASJC Scopus subject areas

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