Effect of laser irradiation on mitochondrial responses of stressed keratinocytes

D. Hawkins Evans, H. Abrahamse

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Photobiomodulation activates the mitochondrial respiratory chain components which initiates signalling cascades that promotes cellular proliferation and cytoprotection. Human keratinocyte cell cultures were modified to induce changes in mitochondrial membrane potential so that the effect of laser irradiation could be investigated. Control conditions included normal keratinocytes and cells treated with p-(tri-fluoromethoxy) phenyl-hydrazone (FCCP), 2-deoxy-D-glucose (2-DOG) and oligomycin. Experimental conditions were used to induce sub-cytotoxic stress namely; keratinocytes grown in media without bovine pituitary extract (BPE), UVC irradiation and tBHP (tert-hydroperoxide). Results confirm that laser irradiation stimulates the mitochondrial respiratory chain to bring about an increase in ATP production, which ultimately results in photobiomodulation to restore homeostasis of stressed cells.

Original languageEnglish
Title of host publicationProceedings of the International Conference of the World Association of Laser Therapy, WALT 2008
PublisherMEDIMOND s.r.l.
Pages21-28
Number of pages8
ISBN (Print)9788875874704
Publication statusPublished - 2008
Event7th International Congress of the World Association of Laser Therapy, WALT 2008 - Sun City, North West Province, South Africa
Duration: 19 Oct 200822 Oct 2008

Publication series

NameProceedings of the International Conference of the World Association of Laser Therapy, WALT 2008

Conference

Conference7th International Congress of the World Association of Laser Therapy, WALT 2008
Country/TerritorySouth Africa
CitySun City, North West Province
Period19/10/0822/10/08

ASJC Scopus subject areas

  • Radiology, Nuclear Medicine and Imaging

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