Effect of hydroxylated and methoxylated coumarins on the regulatory properties of phenylalanine ammonia-lyase from Citrus sinensis

Ian A. Dubery

doi:10.1016/0031-9422(90)83015-S

Effect of hydroxylated and methoxylated coumarins on the regulatory properties of phenylalanine ammonia-lyase from Citrus sinensis

Ian A. Dubery

Biochemistry

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The effects of hydroxylated and methoxylated coumarin derivatives, occurring in γ-irradiated citrus flavedo, on phenylalanine ammonia-lyase from Citrus sinensis were investigated. Umbelliferone and scopoletin inhibited the enzyme by increasing the negative cooperativity between the substrate binding sites. Esculetin, scoparone and fraxetin activated the enzyme, with fraxetin effecting a decrease in negative cooperativity. The coumarin derivatives affected the V_max but not the K_m kinetic parameter.

Original language	English
Pages (from-to)	2107-2108
Number of pages	2
Journal	Phytochemistry
Volume	29
Issue number	7
DOIs	https://doi.org/10.1016/0031-9422(90)83015-S
Publication status	Published - 1990

Keywords

Citrus sinensis
Rutaceae
coumarins.
phenylalanine ammonia-lyase

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

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10.1016/0031-9422(90)83015-S

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title = "Effect of hydroxylated and methoxylated coumarins on the regulatory properties of phenylalanine ammonia-lyase from Citrus sinensis",

abstract = "The effects of hydroxylated and methoxylated coumarin derivatives, occurring in γ-irradiated citrus flavedo, on phenylalanine ammonia-lyase from Citrus sinensis were investigated. Umbelliferone and scopoletin inhibited the enzyme by increasing the negative cooperativity between the substrate binding sites. Esculetin, scoparone and fraxetin activated the enzyme, with fraxetin effecting a decrease in negative cooperativity. The coumarin derivatives affected the Vmax but not the Km kinetic parameter.",

keywords = "Citrus sinensis, Rutaceae, coumarins., phenylalanine ammonia-lyase",

author = "Dubery, {Ian A.}",

year = "1990",

doi = "10.1016/0031-9422(90)83015-S",

language = "English",

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pages = "2107--2108",

journal = "Phytochemistry",

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T1 - Effect of hydroxylated and methoxylated coumarins on the regulatory properties of phenylalanine ammonia-lyase from Citrus sinensis

AU - Dubery, Ian A.

PY - 1990

Y1 - 1990

N2 - The effects of hydroxylated and methoxylated coumarin derivatives, occurring in γ-irradiated citrus flavedo, on phenylalanine ammonia-lyase from Citrus sinensis were investigated. Umbelliferone and scopoletin inhibited the enzyme by increasing the negative cooperativity between the substrate binding sites. Esculetin, scoparone and fraxetin activated the enzyme, with fraxetin effecting a decrease in negative cooperativity. The coumarin derivatives affected the Vmax but not the Km kinetic parameter.

AB - The effects of hydroxylated and methoxylated coumarin derivatives, occurring in γ-irradiated citrus flavedo, on phenylalanine ammonia-lyase from Citrus sinensis were investigated. Umbelliferone and scopoletin inhibited the enzyme by increasing the negative cooperativity between the substrate binding sites. Esculetin, scoparone and fraxetin activated the enzyme, with fraxetin effecting a decrease in negative cooperativity. The coumarin derivatives affected the Vmax but not the Km kinetic parameter.

KW - Citrus sinensis

KW - Rutaceae

KW - coumarins.

KW - phenylalanine ammonia-lyase

UR - http://www.scopus.com/inward/record.url?scp=0003178413&partnerID=8YFLogxK

U2 - 10.1016/0031-9422(90)83015-S

DO - 10.1016/0031-9422(90)83015-S

M3 - Article

AN - SCOPUS:0003178413

SN - 0031-9422

VL - 29

SP - 2107

EP - 2108

JO - Phytochemistry

JF - Phytochemistry

IS - 7

ER -

Effect of hydroxylated and methoxylated coumarins on the regulatory properties of phenylalanine ammonia-lyase from Citrus sinensis

Abstract

Keywords

ASJC Scopus subject areas

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