E. coli Ada regulatory protein repairs the Sp diastereoisomer of alkylated DNA

Michael R. Hamblin; Barry V.L. Potter

doi:10.1016/0014-5793(85)81047-4

E. coli Ada regulatory protein repairs the Sp diastereoisomer of alkylated DNA

Michael R. Hamblin
, Barry V.L. Potter

University of Leicester

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Using HPLC and ³¹P NMR spectroscopy on a chemically synthesized asymmetric mixture of the diastereoisomers of thymidyl(3' → 5')thymidyl-O-methyl phosphate absolute configuration has been correlated with Chromatographic mobility. The methyl phosphotriester system in alkylated DNA which is repaired by the Ada regulatory protein of E. coli has consequently been established to possess the S_p configuration.

Original language	English
Pages (from-to)	315-317
Number of pages	3
Journal	FEBS Letters
Volume	189
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(85)81047-4
Publication status	Published - 23 Sept 1985
Externally published	Yes

Keywords

Configurational analysis
HPLC
P-NMR
Phosphotriester repair

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(85)81047-4

Cite this

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abstract = "Using HPLC and 31P NMR spectroscopy on a chemically synthesized asymmetric mixture of the diastereoisomers of thymidyl(3' → 5')thymidyl-O-methyl phosphate absolute configuration has been correlated with Chromatographic mobility. The methyl phosphotriester system in alkylated DNA which is repaired by the Ada regulatory protein of E. coli has consequently been established to possess the Sp configuration.",

keywords = "Configurational analysis, HPLC, P-NMR, Phosphotriester repair",

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AU - Hamblin, Michael R.

AU - Potter, Barry V.L.

PY - 1985/9/23

Y1 - 1985/9/23

N2 - Using HPLC and 31P NMR spectroscopy on a chemically synthesized asymmetric mixture of the diastereoisomers of thymidyl(3' → 5')thymidyl-O-methyl phosphate absolute configuration has been correlated with Chromatographic mobility. The methyl phosphotriester system in alkylated DNA which is repaired by the Ada regulatory protein of E. coli has consequently been established to possess the Sp configuration.

AB - Using HPLC and 31P NMR spectroscopy on a chemically synthesized asymmetric mixture of the diastereoisomers of thymidyl(3' → 5')thymidyl-O-methyl phosphate absolute configuration has been correlated with Chromatographic mobility. The methyl phosphotriester system in alkylated DNA which is repaired by the Ada regulatory protein of E. coli has consequently been established to possess the Sp configuration.

KW - Configurational analysis

KW - HPLC

KW - P-NMR

KW - Phosphotriester repair

UR - https://www.scopus.com/pages/publications/0021930112

U2 - 10.1016/0014-5793(85)81047-4

DO - 10.1016/0014-5793(85)81047-4

M3 - Article

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JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

E. coli Ada regulatory protein repairs the Sp diastereoisomer of alkylated DNA

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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