Differential metabolic reprogramming in paenibacillus alvei-primed sorghum bicolor seedlings in response to fusarium pseudograminearum infection

René Carlson, Fidele Tugizimana, Paul A. Steenkamp, Ian A. Dubery, Nico Labuschagne

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Metabolic changes in sorghum seedlings in response to Paenibacillus alvei (NAS-6G6)-induced systemic resistance against Fusarium pseudograminearum crown rot were investigated by means of untargeted ultra-high performance liquid chromatography-high definition mass spectrometry (UHPLC-HDMS). Treatment of seedlings with the plant growth-promoting rhizobacterium P. alvei at a concentration of 1 × 108 colony forming units mL−1 prior to inoculation with F. pseudograminearum lowered crown rot disease severity significantly at the highest inoculum dose of 1 × 106 spores mL−1 . Intracellular metabolites were subsequently methanol-extracted from treated and untreated sorghum roots, stems and leaves at 1, 4 and 7 days post inoculation (d.p.i.) with F. pseudograminearum. The extracts were analysed on an UHPLC-HDMS platform, and the data chemometrically processed to determine metabolic profiles and signatures related to priming and induced resistance. Significant treatment-related differences in primary and secondary metabolism post inoculation with F. pseudograminearum were observed between P. alvei-primed versus naïve S. bicolor seedlings. The differential metabolic reprogramming in primed plants comprised of a quicker and/or enhanced upregulation of amino acid-, phytohormone-, phenylpropanoid-, flavonoid-and lipid metabolites in response to inoculation with F. pseudograminearum.

Original languageEnglish
Article number150
JournalMetabolites
Volume9
Issue number7
DOIs
Publication statusPublished - Jul 2019

Keywords

  • Crown rot
  • Fusarium pseudograminearum
  • Induced systemic resistance
  • LC-MS
  • PGPR
  • Paenibacillus alvei
  • Phytoalexin
  • Priming
  • Sorghum bicolor

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology

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