Differential display profiling of the Nicotiana response to LPS reveals elements of plant basal resistance

Natasha M. Sanabria, Ian A. Dubery

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

The identification of cDNAs, representing up-regulated genes induced by lipopolysaccharides from Burkholderia cepacia, was achieved by differential display of mRNAs isolated from tobacco cells. In addition to up-regulation of superoxide dismutase, involved in the production of the signalling and defense molecule, hydrogen peroxide; differentially expressed cDNAs, indicative of the operation of an innate immune recognition system and expression of basal resistance, were identified. These include homologs to a receptor-like protein kinase; a binding protein for the type III secreted effector protein, harpin; a virus resistance N gene; an endogenous pararetrovirus and the Pto kinase. The altered gene expression may be responsible for activation of surveillance mechanisms and enhancement of the non-self recognition capacity. The putative roles of these transcripts in LPS-induced responses are discussed in relation to emerging concepts of innate immunity.

Original languageEnglish
Pages (from-to)1001-1007
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume344
Issue number3
DOIs
Publication statusPublished - 9 Jun 2006

Keywords

  • Basal resistance
  • Defense response
  • Innate immunity
  • Lipopolysaccharides (LPS)
  • Pathogen-associated molecular pattern molecules (PAMPs)

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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