Development of pulsed blue light technologies for bacterial biofilm disruption

Violet V. Bumah; Daniela Masson-Meyers; Dawn Castel; Chris Castel; Chukuka S. Enwemeka

doi:10.1117/12.2510699

Development of pulsed blue light technologies for bacterial biofilm disruption

Violet V. Bumah
, Daniela Masson-Meyers
, Dawn Castel
, Chris Castel
, Chukuka S. Enwemeka

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

4 Citations (Scopus)

Abstract

In recent studies, we showed that pulsed blue light is more potent in suppressing bacterial growth than continuous wave blue light. The potency of pulsed blue light makes it a viable antimicrobial for suppressing bacteria growth in biofilms, where the protective cover of the biofilm makes it is tougher to suppress bacteria. Consequently, we studied the efficacy of pulsed 450 nm light in suppressing the growth of MRSA and P. acnes biofilms. The results showed 100% bacterial suppression in planktonic cultures of MRSA irradiated with 7.6 J/cm² three times a day, using 3 mW/cm² irradiance, and in P. acnes planktonic cultures irradiated with 5 J/cm² thrice daily for 3 days, using 2 mW/cm² irradiance. However, a similar 100% suppression was not attained in MRSA or P. acnes biofilms irradiated thrice daily for 3 days at various fluences; but LIVE/DEAD assay showed a degree of bacterial suppression, with more live cells in controls than irradiated biofilms, and more dead cells in irradiated than control biofilms. In addition, while control biofilms had intact biofilm networks, irradiated biofilms had disrupted biofilm. The higher the dose, the more bacterial suppression and biofilm disruption. These findings confirm our previous reports that 100% bacterial suppression is attainable with pulsed blue light, and suggests further modification of the treatment protocol in order to achieve 100% bacterial suppression in biofilms.

Original language	English
Title of host publication	Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II
Editors	Tianhong Dai, Jurgen Popp, Mei X. Wu
Publisher	SPIE
ISBN (Electronic)	9781510623682
DOIs	https://doi.org/10.1117/12.2510699
Publication status	Published - 2019
Externally published	Yes
Event	Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II 2019 - San Francisco, United States Duration: 4 Feb 2019 → 5 Feb 2019

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	10863
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II 2019
Country/Territory	United States
City	San Francisco
Period	4/02/19 → 5/02/19

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Antimicrobial therapy
Biofilm formation
Methicillin-resistant Staphylococcus aureus
Printed LEDs
Propionibacterium acnes
Pulsed blue light

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.2510699

Cite this

Bumah, V. V., Masson-Meyers, D., Castel, D., Castel, C., & Enwemeka, C. S. (2019). Development of pulsed blue light technologies for bacterial biofilm disruption. In T. Dai, J. Popp, & M. X. Wu (Eds.), Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II Article 108630U (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10863). SPIE. https://doi.org/10.1117/12.2510699

@inproceedings{99b80a6f93914e6685c981f55293d12a,

title = "Development of pulsed blue light technologies for bacterial biofilm disruption",

abstract = "In recent studies, we showed that pulsed blue light is more potent in suppressing bacterial growth than continuous wave blue light. The potency of pulsed blue light makes it a viable antimicrobial for suppressing bacteria growth in biofilms, where the protective cover of the biofilm makes it is tougher to suppress bacteria. Consequently, we studied the efficacy of pulsed 450 nm light in suppressing the growth of MRSA and P. acnes biofilms. The results showed 100\% bacterial suppression in planktonic cultures of MRSA irradiated with 7.6 J/cm2 three times a day, using 3 mW/cm2 irradiance, and in P. acnes planktonic cultures irradiated with 5 J/cm2 thrice daily for 3 days, using 2 mW/cm2 irradiance. However, a similar 100\% suppression was not attained in MRSA or P. acnes biofilms irradiated thrice daily for 3 days at various fluences; but LIVE/DEAD assay showed a degree of bacterial suppression, with more live cells in controls than irradiated biofilms, and more dead cells in irradiated than control biofilms. In addition, while control biofilms had intact biofilm networks, irradiated biofilms had disrupted biofilm. The higher the dose, the more bacterial suppression and biofilm disruption. These findings confirm our previous reports that 100\% bacterial suppression is attainable with pulsed blue light, and suggests further modification of the treatment protocol in order to achieve 100\% bacterial suppression in biofilms.",

keywords = "Antimicrobial therapy, Biofilm formation, Methicillin-resistant Staphylococcus aureus, Printed LEDs, Propionibacterium acnes, Pulsed blue light",

author = "Bumah, \{Violet V.\} and Daniela Masson-Meyers and Dawn Castel and Chris Castel and Enwemeka, \{Chukuka S.\}",

note = "Publisher Copyright: {\textcopyright} 2019 SPIE.; Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II 2019 ; Conference date: 04-02-2019 Through 05-02-2019",

year = "2019",

doi = "10.1117/12.2510699",

language = "English",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "SPIE",

editor = "Tianhong Dai and Jurgen Popp and Wu, \{Mei X.\}",

booktitle = "Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II",

address = "United States",

}

Bumah, VV, Masson-Meyers, D, Castel, D, Castel, C & Enwemeka, CS 2019, Development of pulsed blue light technologies for bacterial biofilm disruption. in T Dai, J Popp & MX Wu (eds), Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II., 108630U, Proceedings of SPIE - The International Society for Optical Engineering, vol. 10863, SPIE, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II 2019, San Francisco, United States, 4/02/19. https://doi.org/10.1117/12.2510699

Development of pulsed blue light technologies for bacterial biofilm disruption. / Bumah, Violet V.; Masson-Meyers, Daniela; Castel, Dawn et al.
Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II. ed. / Tianhong Dai; Jurgen Popp; Mei X. Wu. SPIE, 2019. 108630U (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10863).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Development of pulsed blue light technologies for bacterial biofilm disruption

AU - Bumah, Violet V.

AU - Masson-Meyers, Daniela

AU - Castel, Dawn

AU - Castel, Chris

AU - Enwemeka, Chukuka S.

PY - 2019

Y1 - 2019

N2 - In recent studies, we showed that pulsed blue light is more potent in suppressing bacterial growth than continuous wave blue light. The potency of pulsed blue light makes it a viable antimicrobial for suppressing bacteria growth in biofilms, where the protective cover of the biofilm makes it is tougher to suppress bacteria. Consequently, we studied the efficacy of pulsed 450 nm light in suppressing the growth of MRSA and P. acnes biofilms. The results showed 100% bacterial suppression in planktonic cultures of MRSA irradiated with 7.6 J/cm2 three times a day, using 3 mW/cm2 irradiance, and in P. acnes planktonic cultures irradiated with 5 J/cm2 thrice daily for 3 days, using 2 mW/cm2 irradiance. However, a similar 100% suppression was not attained in MRSA or P. acnes biofilms irradiated thrice daily for 3 days at various fluences; but LIVE/DEAD assay showed a degree of bacterial suppression, with more live cells in controls than irradiated biofilms, and more dead cells in irradiated than control biofilms. In addition, while control biofilms had intact biofilm networks, irradiated biofilms had disrupted biofilm. The higher the dose, the more bacterial suppression and biofilm disruption. These findings confirm our previous reports that 100% bacterial suppression is attainable with pulsed blue light, and suggests further modification of the treatment protocol in order to achieve 100% bacterial suppression in biofilms.

AB - In recent studies, we showed that pulsed blue light is more potent in suppressing bacterial growth than continuous wave blue light. The potency of pulsed blue light makes it a viable antimicrobial for suppressing bacteria growth in biofilms, where the protective cover of the biofilm makes it is tougher to suppress bacteria. Consequently, we studied the efficacy of pulsed 450 nm light in suppressing the growth of MRSA and P. acnes biofilms. The results showed 100% bacterial suppression in planktonic cultures of MRSA irradiated with 7.6 J/cm2 three times a day, using 3 mW/cm2 irradiance, and in P. acnes planktonic cultures irradiated with 5 J/cm2 thrice daily for 3 days, using 2 mW/cm2 irradiance. However, a similar 100% suppression was not attained in MRSA or P. acnes biofilms irradiated thrice daily for 3 days at various fluences; but LIVE/DEAD assay showed a degree of bacterial suppression, with more live cells in controls than irradiated biofilms, and more dead cells in irradiated than control biofilms. In addition, while control biofilms had intact biofilm networks, irradiated biofilms had disrupted biofilm. The higher the dose, the more bacterial suppression and biofilm disruption. These findings confirm our previous reports that 100% bacterial suppression is attainable with pulsed blue light, and suggests further modification of the treatment protocol in order to achieve 100% bacterial suppression in biofilms.

KW - Antimicrobial therapy

KW - Biofilm formation

KW - Methicillin-resistant Staphylococcus aureus

KW - Printed LEDs

KW - Propionibacterium acnes

KW - Pulsed blue light

UR - https://www.scopus.com/pages/publications/85072197268

U2 - 10.1117/12.2510699

DO - 10.1117/12.2510699

M3 - Conference contribution

AN - SCOPUS:85072197268

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II

A2 - Dai, Tianhong

A2 - Popp, Jurgen

A2 - Wu, Mei X.

PB - SPIE

T2 - Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II 2019

Y2 - 4 February 2019 through 5 February 2019

ER -

Bumah VV, Masson-Meyers D, Castel D, Castel C, Enwemeka CS. Development of pulsed blue light technologies for bacterial biofilm disruption. In Dai T, Popp J, Wu MX, editors, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II. SPIE. 2019. 108630U. (Proceedings of SPIE - The International Society for Optical Engineering). doi: 10.1117/12.2510699

Development of pulsed blue light technologies for bacterial biofilm disruption

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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